Sequences from confirmation amplicon


Confirmation sequences for line 340D10 and hit At5g05960 are shown.
Line ID 340D10
Locus At5g05960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1790645
Primers used for PCR KN30 - 8474, show primer details
   
Sequence Name 30-K103250-0022-340-D10C-8409
GenBank Accession LN499677 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K103250-0022-340-D10C-8409
GAGCCTTTACTTTACATGCAAAATTGCAGCTGCAATGAAATAGAAAAAGTTAATAAGCTT
TTTATGTCATTACCTCCACATTTGTAACCGACGGGACGATTAGCGATGTTGCAGCGTTTA
GGAATAGTCATCGAGATCTCTGGCTTTGCTCCGGAGCTCCTAGCTGTGGAAGAAAGCATG
ACAGCACAAAGGCATTTTGGATTCTGCCCCATCTGTTTCACTCGAGCACAGCAAATTGCT
GACACTTTAGCAGATGTGTCTTGTGCAGCCATTGCACAAGGAGCCAACTTTATGGCTTCT
CTGTCTGGAGGATTTCTCCCACATTCTCCAGCCCCATAAACCATGGTTTTAAGGCTGTAG
ATGCAGAGAACTAGGAGGAAAATGCGAGATAAAACATGGGTTTCCATTCTTGAGTGATGA
GTTG
   
Sequence Name 78-K103251-0022-340-D10C-KN30
GenBank Accession LN513675 [GenBank]
Sequencing primer gene specific primer, KN30: 5'-CAACTCATCACTCAAGAATGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K103251-0022-340-D10C-KN30
TTCTCTGCATCTACGCCTTAAACCATGGTTTATGGGGCTGGAGAATGTGGGAGAAATCCT
CCAGACAGAGAAGCCATAAAGTTGGCTCCTTGTGCAATGGCTGCACAAGACACATCTGCT
AAAGTGTCAGCAATTTGCTGTGCTCGAGTGAAACAGATGGGGCAGAATCCAAAATGCCTT
TGTGCTGTCATGCTTTCTTCCACAGCTAGGAGCTCCGGAGCAAAGCCAGAGATCTCGATG
ACTATTCCTAAACGCTGCAACATCGCTNATCGTCCCGTCGGTTACAAATGTGGAGGTAAT
GACATAAAAAGCTTATTAACTTTTTCTATTTCATTGCAGCTGCAATTTTGCATGTAAATG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA

Last Updated on 06.12.2018 16:11