Sequences from confirmation amplicon


Confirmation sequences for line 340F02 and hit At5g56060 are shown.
Line ID 340F02
Locus At5g56060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22704643
Primers used for PCR CL87 - 8474, show primer details
   
Sequence Name 57-K032928-022-340-F02C-8409
GenBank Accession LN509327 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K032928-022-340-F02C-8409
GCCATTTACAATTGAATATGATGTCCATCTTCATCTTTCAGCATCTTTCTTGCACTCTCC
AGAAATCCTCTGACGAGTCCTTGGTGTAACCTGTAAATTGTACACGAAAACGCATATGTT
GAAAATCAACAAGAATAAACAAAAAGAGAAGAAATACAAAAGAAACTTACATAATGAAGA
AGATGTCGTGCTCTGAACCAAAACCTAATCCTGAATGGGGAAAATTAAAGATGATTCTAT
CGTATCGACCTAAGCGATAGTCTGAGGACATGGAGTGGACGTTAACACCGTGGACCACTG
TGCACCCAAACAACTCCAACCCTTCCACATTTGCTTTTCCATCAGTGTACTTAATCCCTA
GCTCCTCTACAACATTCATCATTAGCTTATAACAATAATCACCAAATTAACTTTCATATC
TTATGTTCGGTGTTTTAGATGTATTATCAGAAGATGATAAAAATAACCAAATATGTAAGA
TGAATTGTGGTGTTAGAAATATTTCTACCTCGAGTGTCAAGAGAGGTCGCAGTGATGTTT
GTGGCAGAACCAAAAACTCTGGCTAGAGACAAAGAAAACGAAAAGTCTCCTTCTCCGACC
AAAAGTATTTTTTGCTTGTTGCT
   
Sequence Name 57-K032928-022-340-F02C-CL87
GenBank Accession LN509328 [GenBank]
Sequencing primer gene specific primer, CL87: 5'-GCAACAATATAGCAACAAGCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K032928-022-340-F02C-CL87
TTTCGTTTTCTTTGTCTCTAGCCGAGTTTTTGGTTCTGCCACAAACATCACTGCGACCTC
TCTTGACACTCGAGGTAGAAATATTTCTAACACCACAATTCATCTTACATATTTGGTTAT
TTTTATCATCTTCTGATAATACATCTAAAACACCGAACATAAGATATGAAAGTTAATTTG
GTGATTATTGTTATAAGCTAATGATGAATGTTGTAGAGGAGCTAGGGATTAAGTACACTG
ATGGAAAAGCAAATGTGGAAGGGTTGGAGTTGTTTGGGTGCACAGTGGTCCACGGTGTTA
ACGTCCACTCCATGTCCTCAGACTATCGCTTAGGTCGATACGATAGAATCATCTTTAATT
TTCCCCATTCAGGATTAGGTTTTGGTTCAGAGCACGACATCTTCTTCATTATGTAAGTTT
CTTTTGTATTTCTTCTCTTTTTGTTTATTCTTGTTGATTTTCAACATATGCGTTTTCGTG
TACAATTTACAGGTTACACCAAGGACTCGTCAGAGGATTTCTGGAGAGTGCAAGAAAGAT
GCTGAAAGATGAAGATGGACATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTAC
Primers used for PCR CL88 - 8474, show primer details
   
Sequence Name 58-K032928-022-340-F02C-CL88
GenBank Accession LN509617 [GenBank]
Sequencing primer gene specific primer, CL88: 5'-TGTCTCTTTACTGGTCTCGTGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K032928-022-340-F02C-CL88
AGGTATCATCGTTGTTAAGTCTGTTTATTTAGGAAAATAGCCAACAATTAATCTCTTTCA
TTACACTATTATAGAATTCTTGATAAATTAATCAACGAACACTATATCTTAAACATGAAA
GTAGAAGCTTTTACGATAGGAAAGTGTTAAGAAGGATACTTTCCTTACTAGATTATCATG
TCTATTCCAAAGGAAGATGCACTAAAATGTACAATGATAATCTAGTAAGGAAAGTCTCCT
TCTTAACACACAGGTAGAGTTACCGTTACTTCCATCCTCCTCTCTCGTTGGAGTAACGGG
GAAGTGCCCATTTGTGACACTCGATCTCCCGTA
   
Sequence Name 58-K032928-022-340-F02C-oriL
GenBank Accession LN509618 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K032928-022-340-F02C-oriL
ATAAGACAACAGACCCATTTAACAGGTGGGGGATTGAGACTCTAGCTGGAGAAAAGGGTT
TGCGTCTAATCGGGGAGATCGAATTTCACAAATGGGCATTTCCAGGTTACTCCAACAAGA
AAGGAGGTGGAAGTAACTGTAACTCTACCTTTCTGTTAAGAAGGATACTTTCCTATCNNN
AAANCTTCTACTTTCATGTTTAATATATANTGTTCATTTATTAATTTATCAANAATTCTA
TAATACTGTAATGAAACAAATTAATTGTTGGCTATTTTCCTAAATAAACAGACTTTAACA
ACGATGATTACCTTTGATCAAAATAAAATAAAAGTCAATTTTTGTTAGCACGAGACCAGT
AAAGAGAC

Last Updated on 06.12.2018 16:11