Sequences from confirmation amplicon


Confirmation sequences for line 340H04 and hit At3g14570 are shown.
Line ID 340H04
Locus At3g14570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4893658
Primers used for PCR VL55 - 8474, show primer details
   
Sequence Name 17-K024207-022-340-H04C-VL55
GenBank Accession LN493692 [GenBank]
Sequencing primer gene specific primer, VL55: 5'-TAATACAACACCTTTGGAGCTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K024207-022-340-H04C-VL55
GCTTCTGTGGTTGCCGTATAATGGTGTTACTGAGTAGTAGAATCAGATGCTCTCTCTGAT
TGGCAACATTTCCATTCTAGCAGCAAAAAAAATTGAAAGTCTGGTCACCATGTGTAATTA
CAGTCTTTACAATACCGATAGCAAGTTCCTGACAAGTACCTGGAACCCAAAGGCATACTG
CAGAAATTCAAATAAATCCAGAAAAGGCTGGTGTCTCTGAAATTCCTCTGGAGGAGGCAA
CCCGCGAGTGTTACGTACAATTGCAACAGCAGCTTTAATCTGTATTTTAGGACATAAAGG
AACATTTCACCATTAAAATTTCAATATGCTGAGTTACATAACTATACTACAATGGAAAAT
CATAATAAGCAAGTGGCAACAATTCTGTTTCCCAATGAGGCCATGTATCTTTATACCTCA
GGCAGATGCATAATTGCTTGGTGTACCCCTCCTTTATCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAA
   
Sequence Name 18-K024207-022-340-H04C-8409
GenBank Accession LN494140 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K024207-022-340-H04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGATAAAGGAGGGGTACACCAAGCAATT
ATGCATCTGCCTGAGGTATAAAGATACATGGCCTCATTGGGAAACAGAATTGTTGCCACT
TGCTTATTATGATTTTCCATTGTAGTATAGTTATGTAACTCAGCATATTGAAATTTTAAT
GGTGAAATGTTCCTTTATGTCCTAAAATACAGATTAAAGCTGCTGTTGCAATTGTACGTA
ACACTCGCGGGTTGCCTCCTCCAGAGGAATTTCAGAGACACCAGCCTTTTCTGGATTTAT
TTGAATTTCTGCAGTATGCCTTTGGGTTCCAGGTACTTGTCAGGAACTTGCTATCGGTAT
TGTAAAGACTGTAATTACACATGGTGACCAGACTTTCAATTTTTTTTGCTGCTAGAATGG
AAATGTTGCCAATCAGAGAGAGCATCTGATTCTACTACTCAGTAACACCATTATACGGCA
ACCACAGAAGCAGTCTTCAGCTCCAAA

Last Updated on 06.12.2018 16:11