Sequences from confirmation amplicon


Confirmation sequences for line 340H07 and hit At1g75660 are shown.
Line ID 340H07
Locus At1g75660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28414726
Primers used for PCR 23lm - 8474, show primer details
   
Sequence Name 31-K113916-0022-340-H07C-23lm
GenBank Accession LN500246 [GenBank]
Sequencing primer gene specific primer, 23lm: 5'-TGCTGTCTTCGTTTTTAAATCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K113916-0022-340-H07C-23lm
TGTTATAGTTTAACTGAATAAAACAATAGTTTGAAAACAAGGTACAATACAAAGCATCTG
TATATAAATGATACACCAAATAAAAAATGTCGAAAAGGGTAGGAAAACTAATGTAACCTA
TAGTTTCTCCCAGCTGCTCTTATTCCCATTAAGCTTAAGACCAAGAGCAGCCCAATTCTA
ATGATACAACAAACACTTCATAACAAACGTATGAAACAAGTTCAACTACACCTGATGGTT
GATACGGTTAGGAGAGATTCAGCAGTAGTACCATCCTGCTCAATATCGATGTCTAGGATT
CCCGCCTTGATCATGATGATGATGACTTCCTCTTCCTCTTCCTCTGCGGTCATGGTCACC
ACCTTGTTGATGATGATGCTGACCATTATACCCTCGTGGGTTATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 03-K113915-0022-340-H07C-8409
GenBank Accession LN487570 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K113915-0022-340-H07C-8409
CCACGTGGGGTAATAAGGGTTCGCATCATCATTCAACAAGGTGGTGACCATGACCGCAGT
GGAAGTGGAAGAGGAAGTTCATCATCATCATGATCAAGGCGGGAATCCTAGACATCGATA
TTGAGCAGGATGGTACTACTGCTGAATCTCTCCTAACCGTATCAACCATCAGGTGTAGTT
GAACTTCGTTTCATACGTTTGTTATGAAGTGTTTGTTGTATCATTAGAATTGGGCTGCTC
TTGGTCTTAAGCTTAATGGGAATAAGAGCAGCTGGGAGAAACTATAGGTTACATTAGTTT
TCCTACCCTTTTCGACATTTTTTATTTGGTGTATCATTTATATACAGATGCTTTGTATTG
TACCTTGTTTTCAAACTATTGTTTTATTCAGTTAAACTATAACATATATGCTTCTTCACT
GGTTCGATCTTTAAACTCTCTGCAAAAGGATTTAAAA

Last Updated on 06.12.2018 16:11