Genotyping and primer details


Genotyping and primer details for 340H07 - At1g75660
Line ID 340H07
Locus At1g75660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28414726 of line 340H07 Chr1:28414726
   
Insertion allele amplicon 8474 - 23lm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23lm: 5'-TGCTGTCTTCGTTTTTAAATCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28415169
   
wt amplicon (over InsPos) 02lz - 23lm
wt amplicon start Chr1:28414284
wt amplicon end Chr1:28415169
wt amplicon size 886 bp
Locus specific primer 23lm: 5'-TGCTGTCTTCGTTTTTAAATCCTT-3'
2nd locus specific primer 02lz: 5'-TTTCTCCTCATTTCTTTAGGCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11