Sequences from confirmation amplicon


Confirmation sequences for line 341B12 and hit At1g45191 are shown.
Line ID 341B12
Locus At1g45191 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17116598
Primers used for PCR BV52 - 8474, show primer details
   
Sequence Name 04-K106147-0022-341-B12C-BV52
GenBank Accession LN487928 [GenBank]
Sequencing primer gene specific primer, BV52: 5'-GGGACTTACCATGTTTGACAAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K106147-0022-341-B12C-BV52
TTTCGGGTTATGGAACCTCTTCCTCTAATGAGACAAGAGGACATAAAAGGGATTCTTTGA
AGTAAAGTTGGAAAAAGCGGTCCCTTAAAAAGAAGACTTACTAGAGATGAGTCTAGACCA
AGAGATGGAGAATCTGAATGTATGTAAGCCACTTCCGGCCATGAGCTGCACATCTTCCTG
CATATTTAGGTAAAAACCTTTGATAACAAGGCCATAGCCAATTTTTCTTGGTGAATATAT
ATATAAGATGTATTGACAGAGAAAGAACCGTATACTTGTGATATCCTTTTCAAGCTATGT
CTCCGTTATCCATTTTACCTGCCAAGTACAAATATTTATTCTCTCCGTTTCACATTATGT
GATGTGTATTGAAGGACTTGTTTTTTTT
   
Sequence Name 20-K106148-0022-341-B12C-8409
GenBank Accession LN495222 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K106148-0022-341-B12C-8409
TTACTGAGTACCATATTTTGTTTTTAATAATATGGGAGACTAGTCTCCCTCATTCTACCA
AGAAAAAAATGGCTTACTGCAGAATATAAATAATCAAAAAAATTAAATACATTAAAGTTT
TGTATGAAGACTTGGAAACATCACTTAAAATACAAAGTAAAAAAAAAAAAAATTCAGGAC
CCATCATTTAAAGTGAAACGGAAAGAGTAAATGTTTGTACTTGGCGGGAAAAAGGGATAA
GGGAAACATACCTGGGGAGGGAAATCACAAGTAAAAGGTTCTTTCCCTGTCTATACAGGT
TATATATATTTCCCCCAAAAAAAATGGGCTATGGCCTTGTTATCAAAGGTTTTTACCTAA
CTATGCAGGAAAATGTGCACCTCAGGGCCAAAACGGGCTTACATACATTCAAATTCCCCA
TCTCTGGGTCAAAACCCATCTCTAGAAAGTCTTCTTTACCAAGGACCGCTTTTTCCAACT
TTACTTCAAAAAATCAAAATTTTGACCTCTGGTCCCATTAAAGGGAAAAGGTTCCTTTAA
CCCAAAGGGTCTACAGTTCTACAAAAATTTCATTCAAAAACTTGTCAAACA

Last Updated on 06.12.2018 16:11