Sequences from confirmation amplicon


Confirmation sequences for line 341D06 and hit At2g44160 are shown.
Line ID 341D06
Locus At2g44160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18264671
Primers used for PCR JZ24 - 8474, show primer details
   
Sequence Name 27-K037808-022-341-D06C-8409
GenBank Accession LN498296 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K037808-022-341-D06C-8409
GCCATTTCAATTGAATATATCCTGCAAATCATACACAAGCACAAGTATCGATCAATCACG
AATCAAAACTCTGTTTGCTATGAGAACAGAGATATAACAGGACTGATTCCTCACCAACAG
TTGGGGAATCAGATCTCTCGGCGTTGACTGATGGTTGGCTATTGATGGTCAAGAAGCCTT
TGGAGTTGACTTTTATCAGTTGCTCGTTTATTATCCTTGTCTCTGGCTGGAGTCCATCTA
ATTCAGACCAGGGACTGCTTTTAAGGTTTCCAAGGCAGAGCTCCTTGAATTTCTGTTATG
TGACAGTTTTTTTGCAAGATTATTGTAAGCGTCTGCATCTTTACAGGTGAAAGTGAGGGA
AATGTGTGGTAGTGAAAAAATTGTTCTACCTCCTGAATATCTTCAACACTTTTCAGTGGG
ACAACCCATTCTTGTTGAAGCTTCTTGTCACGTGCTCGCGGACGTGAGAACTGACAACAA
AAGCATTTAGTTCAGCAAACAAGCTCACAAGAAGATGTTACATTTAACAATCTAGAGCAA
ATCAAAAAGACCAATCGAAGAAATACCCTTCAAAACCCCTTTGTTAT
   
Sequence Name 19-K037808-022-341-D06C-JZ24
GenBank Accession LN494673 [GenBank]
Sequencing primer gene specific primer, JZ24: 5'-TTTCAAGATTTCATAACAAAGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K037808-022-341-D06C-JZ24
TCTTCGATATGGTCTTTTTGATTTGCTCTAGATTGTTAAATGTAACATCTTCGTTGTGAG
CTTGTTTGCTGAACTAAATGCTTTTGTTGTCAGTTCTCACCTCCGCGAGCACGTGACAAG
AAGCTTCAACAAGAATGGGTTGTCCCACTGAAAAGTGTTGAAGATATTCAGGAGGTAGAA
CAATTTTTTCACTACCACACATTTCCCTCACTTTCACCTGTAAAGATGCAGACGCTTACA
ATAATCTTGCAAAAAAACTGTCACATAACAAAAATTCAAGGAGCTCTGCCTTGGAAACCT
TAAAAGCAGTCCCTGGTCTGAATTAGATGGACTCCAGCCAGAGACAAGGATAATAAACGA
GCAACTGATAAAAGTCAACTCCAAAGGCTTCTTGACCATCAATAGCCAACCATCAGTCAA
CGCCGAGAGATCTGATTCCCCAACTGTTGGTGAGGAATCAGTCCTGTTATATCTCTGTTC
TCATAGCAAACAGAGTTTTGATTCGTGATTGATCGATACTTGTGCTTGTGTATGATTTGC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTC

Last Updated on 06.12.2018 16:11