Sequences from confirmation amplicon


Confirmation sequences for line 342B12 and hit At2g43330 are shown.
Line ID 342B12
Locus At2g43330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18001229
Primers used for PCR NS05 - 8474, show primer details
   
Sequence Name 16-K020068-022-342-B12C-8409
GenBank Accession LN493237 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K020068-022-342-B12C-8409
CATGTAGATTTCCCGGACATGAAGCCATTGGGTTTGACTGTCACTGCCGGAATCGGTGGC
CTTCTGTTCGGTTATGATACAGGTCTCTCTCTATCTCTCTTACTCACCAATTATGATGTT
TTAGGCGTTTGATTTGATTTCCTTATTGACCCTTTTGTAGAAATTCTGTAAATTGAATCG
AAAGTGGATTCTTTTAGTCTGCATTACTTGTCTCATTGGATTTTACTATATTCAGAAATT
GGTTTGTGTTTGGGTTATTTAGATTTTAGTCAAAGCCTTTAAGAAATGTTTCCTTTTACA
GGTGTAATTTCCGGTGCCCTTTTGTACATTAAGGATGATTTCGAAGTTGTTAAGCAGAGC
AGTTTCTTACAGGTATATAATGTTTCTAGCTTTACCTCTTCCAAGCTGGTAACTTTCAGT
GTTAGTAGTGATTTATGAAGAATTTGATCTTTGTAGGAAACTATTGTAAGTATGGCTTTA
GTTGGTGCCATGATTGGTGCTGCACTGGAGTTGGATCAATGACTACTACGGTCGTAAAAA
GGCGACTCTTTTTGCAGATGTTGTCTTTGCAGCTGGAGCTATCGTTATGGCTGCTGCTCC
TGATCCGTATGTTTTG
   
Sequence Name 15-K020068-022-342-B12C-NS05
GenBank Accession LN492768 [GenBank]
Sequencing primer gene specific primer, NS05: 5'-AGACGACCCGATATCAAAACATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K020068-022-342-B12C-NS05
CAGCAGCCATAACGATAGCTCCAGCTGCAAAGACAACATCTGCAAAAAGAGTCGCCTTTT
TACGACCGTAGTAGTCATTGATCCAACCTCCAGCTGCAGCACCAATCATGGCACCAACTA
AAGCCATACTTACAATAGTTTCCTACAAAGATCAAATTCTTCATAAATCACTACTAACAC
TGAAAGTTACCAGCTTGGAAGAGGTAAAGCTAGAAACATTATATACCTGTAAGAAACTGC
TCTGCTTAACAACTTCGAAATCATCCTTAATGTACAAAAGGGCACCGGAAATTACACCTG
TAAAAGGAAACATTTCTTAAAGGCTTTGACTAAAATCTAAATAACCCAAACACAAACCAA
TTTCTGAATATAGTAAAATCCAATGAGACAAGTAATGCAGACTAAAAGAATCCACTTTCG
ATTCAATTTACAGAATTTCTACAAAAGGGTCAATAAGGAAATCAAATCAAACGCCTAAAA
CATCATAATTGGTGAGTAAGAGAGATAGAGAGAGACCTGTATCATAACCGAACAGAAGGC
CACCGATTCCGGCAGTGACAGTCAAACCCAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11