Sequences from confirmation amplicon


Confirmation sequences for line 342G06 and hit At3g44060 are shown.
Line ID 342G06
Locus At3g44060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15825170
Primers used for PCR 52pq - 8474, show primer details
   
Sequence Name 33-K115371-0022-342-G06C-52pq
GenBank Accession LN501121 [GenBank]
Sequencing primer gene specific primer, 52pq: 5'-AAAAGCATTAAGAAACTTTCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K115371-0022-342-G06C-52pq
ATTCTTACCTTGACACGCCAGTGTTGTCGACCTCTACTACTCTGATTCCCCTCGGCATAA
CGCTCCACTTTTTCATTTAGATTCACTTGCGAAAGTTACATTGGACCTTCATTTCATAGA
AAATAACAATCGCGAGGTGCAAAATGACGCTGATGTGAAAAATCTCATTAGGGAGATATG
CAACGTCAAGACCCTTCACTTGACTTGTTCTACAGTCGAGGTAAGTTCTTAATGTATCGT
CTCTCATGATTCTTTCTGTTAAAAAGCTGTCCAAGTTCAAACATTCTTTGTGTGTGTGTT
ATCAGGTGATTTCCGTATACTGCAAAGGTGGACTACCAATGTTCAACAACCTTGTTGAGT
TAGTGTTTTCGAGTAAAAAGGAAGGTTGGAGAGTGCTTCTGCCTCTTCTGCTAGAGAATT
CTCCAAACCTTGAAACTCTGGTTCTCTCGGTATGAAGAAAACCCTGAACAAATCTTCATT
TATATGTATGATTGTGACATGTGATTAAAGTAGTATCTTCTCTTGATGCTTCAGGATCTG
CATCGTTACACATTTGGACGAAGACATCGGTTTGTCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 13-K115370-0022-342-G06C-8409
GenBank Accession LN492173 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K115370-0022-342-G06C-8409
TTTACATTGAATATATCCTGACAAACCGATGTCTTCGTCCAAATGTGTAACGATGCAGAT
CCTGAAGCATCAAGAGAAGATACTACTTTAATCACATGTCACAATCATACATATAAATGA
AGATTTGTTCAGGGTTTTCTTCATACCGAGAGAACCAGAGTTTCAAGGTTTGGAGAATTC
TCTAGCAGAAGAGGCAGAAGCACTCTCCAACCTTCCTTTTTACTCGAAAACACTAACTCA
ACAAGGTTGTTGAACATTGGTAGTCCACCTTTGCAGTATACGGAAATCACCTGATAACAC
ACACACAAAGAATGTTTGAACTTGGACAGCTTTTTAACAGAAAGAATCATGAGAGACGAT
ACATTAAGAACTTACCTCGACTGTAGAACAAGTCAAGTGAAGGGTCTTGACGTTGCATAT
CTCCCTAATGAGATTTTTCACATCAGCGTCATTTTGCACCTCGCGATTGTTATTTTCTAT
GAAATGAAGGTCCAATGTAACTTTCGCAAGTGAATCTAAATGAAAAAGTGGAGCGTTATG
CCGAGGGGAATCAGAGTAGTAGAGGTCGACAACACTTGGCGTGTCAAGTGTAAGAATATA
ACCAGCACCATAATAACCACTGTTAACGCTAAGTGAAAGT

Last Updated on 06.12.2018 16:11