Sequences from confirmation amplicon


Confirmation sequences for line 343B08 and hit At3g13784 are shown.
Line ID 343B08
Locus At3g13784 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4530498
Primers used for PCR RQ01 - 8474, show primer details
   
Sequence Name 07-K026965-022-343-B08C-8409
GenBank Accession LN489116 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K026965-022-343-B08C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGATACGTCACCGGTT
ATTTACATGATGACGTTATACCGGATTCATTAATTCAGTTTCACTTGGTTATCTTTTTAT
TCCTTTCTCTATAATTTGTGCACATTGTCAAATCTTTTCACTCATTTTGTTTGTTTGTTT
ATGTTGGGGCTTCGTTTCAATGCTACAAGATCCTAACGGTAAGTTATTATTGCTCTCTCG
TTTGTTTTATAAATTACGTATAAGTAAGCATGGATATAACATATAAGTAATATGTATGTT
TATGTACATAAAATTGGACAGGGCCTATGATATACAAAGGAATCTATCATCTATTCTATC
AATGGAACCAAAACGGCGCCGTCATGGACGTTAACAAAACCGTATGGGGCCATGCAACAT
CAACGGATCTAATCA
   
Sequence Name 08-K026965-022-343-B08C-RQ01
GenBank Accession LN489578 [GenBank]
Sequencing primer gene specific primer, RQ01: 5'-GTGATCCAGTTGATTAGATCCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K026965-022-343-B08C-RQ01
GATGTTGCATGGCCCCATACGGTTTTGTTAACGTCCATGACGGCGCCGTTTTGGTTCCAT
TGATAGAATAGATGATAGATTCCTTTGTATATCATAGGCCCTGTCCAATTTTATGTACAT
AAACATACATATTACTTATATGTTATATCCATGCTTACTTATACGTAATTTATAAAACAA
ACGAGAGAGCAATAATAACTTACCGTTAGGATCTTGTAGCATTGAAACGAAGCCCCAACA
TAAACAAACAAACAAAATGAGTGAAAAGATTTGACAATGTGCACAAATTATAGAGAAAGG
AATAAAAAGATAACCAAGTGAAACTGAATTAATGAATCCGGTATAACGTCATCATGTAAA
TAACCGGTGACGTATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11