Sequences from confirmation amplicon


Confirmation sequences for line 343C11 and hit At1g66440 are shown.
Line ID 343C11
Locus At1g66440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24784044
Primers used for PCR 30ns - 8474, show primer details
   
Sequence Name 49-K114626-0022-343-C11C-30ns
GenBank Accession LN506927 [GenBank]
Sequencing primer gene specific primer, 30ns: 5'-ATTTTTCTCGCAAAATACAACACC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K114626-0022-343-C11C-30ns
CGCGACCGATAAAGCCAACACTCATGATTTATGTTCAGCTTGCCCATGGTTGTTCACCAC
CNGGTTTCTTCTATGAGTGCCACAGAGAAGGATGTCGTTTTAATCTACATGTGCGGTGCG
GGACAATTTCTGAACCATTAGTTCATGGAAGTCATAGGCATCCTTTATTCCTAACATCTA
AACCAGGAGATTGGAGAACATGTAGTGTTTGCAAAGAGTCAAGCCGTTATGTCACACATG
AAACATTTAATTGCGTTCAATGTGTCTTTGCTTTGTGTTTCAAGTGTGCTGCTATACCTC
AGAAGGTGAGGTATAAGCATGATAATCATGTGCTCATTCTTTCTTATGGGAAGGAGATCG
AGACAAGTACCATGGCGTATTGTGATGAAATAAATCCGAAAGGGCAGTTTTATAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
   
Sequence Name 49-K114625-0022-343-C11C-8409
GenBank Accession LN506926 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K114625-0022-343-C11C-8409
ACATTATAAACTGCCCTTTCGGATTTATTTCATCACAATACGCCATGGTACTTGTCTCGA
TCTCCTTCCCATAAGAAAGAATGAGCACATGATTATCATGCTTATACCTCACCTTCTGAG
GTATAGCAGCACACTTGAAACACAAAGCAAAGACACATTGAACGCAATTAAATGTTTCAT
GTGTGACATAACGGCTTGACTCTTTGCAAACACTACATGTTCTCCAATCTCCTGGTTTAG
ATGTTAGGAATAAAGGATGCCTATGACTTCCATGAACTAATGGTTCAGAAATTGTCCCGC
ACCGCANATGTAGATTAAAACGACATCCTTCTCTGTGGCACTCATAGAAGAAACCGGTGG
TGAACAACCATGGGCAAGCTGAACATAAATCATGAGTGTTGGCTTTATCGGTCGCGGAAG
TCACTAAAGTGAGCAGATGTGGATGTATCGGGTGTTGTATTTAGCGAGAAAAATACAACA
CCCGATACATCCACATCTGCTCACTTTAGTGACTTCCGCGACCGATAAAGCCAACACTCA
TGATTTATGTTCAGCTTGNACATGGTTGT

Last Updated on 06.12.2018 16:11