Sequences from confirmation amplicon


Confirmation sequences for line 343D06 and hit At1g28130 are shown.
Line ID 343D06
Locus At1g28130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9827837
Primers used for PCR 15qk - 8474, show primer details
   
Sequence Name 18-K115635-0022-343-D06C-15qk
GenBank Accession LN494441 [GenBank]
Sequencing primer gene specific primer, 15qk: 5'-ACGCAGTGACACAAGCTAAACTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115635-0022-343-D06C-15qk
AAGCTACGCAGAACACGTTCATCAATCCCAGGGCATTACGTTGCTCTTCTGGGAGCTAAA
GCCACGTCACAGCAATGACCCACCAAAGCTTGACGACAAGACAATGGAGGATTGTTGCTC
TGAGGTTGAGGATTGTTTGGATTACGTCTACAGGAGATGCAGGAACAGGGACAAGTCGAT
TGGGCCATTGGAGATAAGAGTGGTGAGTTTGGGCACGTTTGATTCGTTAATGGATTTTTG
TGTCTCACAAGGATCATCTTTGAATCAGTATAAGACTCCAAGATGTGTTAAATCTGGAGG
AGCTCTTGAGATTCTTGATTCCAGAGTTATTGGGAGGTTCTTGATTTCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 04-K115634-0022-343-D06C-8409
GenBank Accession LN488048 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115634-0022-343-D06C-8409
TGAAATCAAGAACCTCCCAATAACTCTGGAATCAAGAATCTCAAGAGCTCCTCCAGATTT
AACACATCTTGGAGTCTTATACTGATTCAAAGATGATCCTTGTGAGACACAAAAATCCAT
TAACGAATCAAACGTGCCCAAACTCACCACTCTTATCTCCAATGGCCCAATCGACTTGTC
CCTGTTCCTGCATCTCCTGTAGACGTAATCCAAACAATCCTCAACCTCAGAGCAACAATC
CTCCATTGTCTTGTCGTCAAGCTTTGGTGGGTCATTGCTGTGACGTGGCTTTAGCTCCCA
GAAGAGCACGTAATGCCCTGGGATTGATGACGTGTCTGCGTAGCTTGTGTACTCCGTGAG
CAAGAGGCTTGAAGGATGTTGAAGATGGTTTAGTTTAG

Last Updated on 06.12.2018 16:11