Sequences from confirmation amplicon


Confirmation sequences for line 343D11 and hit At2g43890 are shown.
Line ID 343D11
Locus At2g43890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18178071
Primers used for PCR 58qa - 8474, show primer details
   
Sequence Name 53-K115305-0022-343-D11C-58qa
GenBank Accession LN508248 [GenBank]
Sequencing primer gene specific primer, 58qa: 5'-GAACTTGGGACAAACATTTTGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K115305-0022-343-D11C-58qa
GAATGTTTGACTTGAATCTTTAATAGGTCCAAATATATTTTGTACGAAAAAGGGTACAAA
CTGAAGTTTCAAGATATATATGGATGAAGACGCAAAACAAAATAACTCGATCACAGAAGG
CAAAAAATACTCTGCGTCCATGACGGTGTAATGTCATCTAAACACACTTCAAATTCCATG
AATTATATATCAAAGACAACCTTGAGGCATCACTACACCAGCCTTAACTCCTTTAATATT
CTTACAAGTCGAAGTAGCGGACCGACCGTTAAACGTTAGCTTAATATCATGCAATCTAAT
CGCTTGGCACGGATTACTGCGACTGCAATCAAACGTTAAAGCCTGTTGCGTCCGCGACGT
CCCTTGAATGTTTCTATACACAACCTGACTAATCTTCACTCCAGAACCCTACAAATATAC
AAGCCAATCAAATCAAGTTTCTAGAATTCCTTTGTATATGTAGGAAGCTAGGTTTCAATT
TCGATTAAGAAATTTACCTGTTTAGGACATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 18-K115304-0022-343-D11C-8409
GenBank Accession LN494432 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115304-0022-343-D11C-8409
TATGTCCTAAACAGGTAAATTTCTTAATCGAAATTGAAACCTAGCTTCCTACATATACAA
AGGAATTCTAGAAACTTGATTTGATTGGCTTGTATATTTGTAGGGTTCTGGAGTGAAGAT
TAGTCAGGTTGTGTATAGAAACATTCAAGGGACGTCGCGGACGCAACAGGCTTTAACGTT
TGATTGCAGTCGCAGTAATCCGTGCCAAGCGATTAGATTGCATGATATTAAGCTAACGTT
TAACGGTCGGTCCGCTACTTCGACTTGTAAGAATATTAAAGGAGTTAAGGCTGGTGTAGT
GATGCCTCAAGGTTGTCTTTGATATATAATTCATGGAATTTGAAGTGTGTTTAGATGACA
TTACACCGTCATGGACGCAGAGTATTTTTTGCCTTCTGTGATCGAGTTATTTTGTTTTGC
GTCTTCATCCATATATATCTTGAAACTTCAGTTTGTACCCTTTTTCGTACAAAATATATT
TGGACCTATTAAAGATTCAAGTCAAACATTCGTTTGATATTTTTTTTATTAATGTAAAGC
GTATTTGAATCAAAAATCAAAATGTT

Last Updated on 06.12.2018 16:11