Sequences from confirmation amplicon


Confirmation sequences for line 343H07 and hit At1g55750 are shown.
Line ID 343H07
Locus At1g55750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20841907
Primers used for PCR 17qk - 8474, show primer details
   
Sequence Name 52-K115459-0022-343-H07C-17qk
GenBank Accession LN507911 [GenBank]
Sequencing primer gene specific primer, 17qk: 5'-ATTGACATTGTGCTCTTTGTATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K115459-0022-343-H07C-17qk
TTAATATAACTTTCCCATACCAGGACCACGGGATCCAGCGTGATGGCACCATGGATGTTG
TTGAACCACAAAATGATCAGTTTAAACGATCACTTTTGCAAGATCTTAATCGTCATGCTG
CTGTTGTATTGGAAGGCAGAAGCATTGGTAAACACCTCTAAGAACCTCATTTGAATCTTT
TTATCTGAATATTTTATAACAGTAGATAGTTCAGGAAGTGTAAACATTTATGTTGGCCTT
TTGACTTTAGTTCTTGGTGGTAAATATACAATAATATTCCTGTAATTGGAAAGCAGATGT
TGAGTCAGAAGATACAAGGATTGTTGCAGAGGCTCTCACACGGGTTAAACAAGGTATGAT
GTTTCATTCTGCTAAACAATGCCACGCTTTTTATTCATCTGACCTTGCTATCCATCTCTA
TTTTACACCCCTGTTAGTAAGCAAAGCTGATGGTGAAACCACCAAGGACGCTNATCAGGA
GAGATTGGAAAGAATGTCCCCTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 03-K115458-0022-343-H07C-8409
GenBank Accession LN487617 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115458-0022-343-H07C-8409
CATTTCATTGAATATATCCTGACGGGGACATTCTTTCCATCTCTCCTGATTAGCGTCCTT
GGTGGTTTCACCATCAGCTTTGCTTACTAACAGGGGTGTAAAATAGAGATGGATAGCAAG
GTCAGATGAATAAAAAGCGTGGCATTGTTTAGCAGAATGAAACATCATACCTTGTTTAAC
CCGTGTGAGAGCCTCTGCAACAATCCTTGTATCTTCTGACTCAACATCTGCTTTCCAATT
ACAGGAATATTATTGTATATTTACCACCAAGAACTAAAGTCAAAAGGCCAACATAAATGT
TTACACTTCCTGAACTATCTACTGTTATAAAATATTCAGATAAAAAGATTCAAATGAGGT
TCTTAGAGGTGTTTACCAATGCTTCTGCCTTCCAATACAACAGCAGCATGACGATTAAGA
TCTTGCAAAAGTGATCGTTTAAACTGATCATTTTGTGGTTCAACAACATCCATGGTGCCA
TCACGCTGGATCCCGTGGTCCTGTGTTATGGGAAAGTTATATTAAGTACGATAAAGTGTA
TTGAAAATAACGTTTCAACCGCATACAAAGAGCAAAATGTCAATA

Last Updated on 06.12.2018 16:11