Sequences from confirmation amplicon


Confirmation sequences for line 344B01 and hit At2g32295 are shown.
Line ID 344B01
Locus At2g32295 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13719600
Primers used for PCR 39sm - 8474, show primer details
   
Sequence Name 43-K116491-0022-344-B01C-39sm
GenBank Accession LN504969 [GenBank]
Sequencing primer gene specific primer, 39sm: 5'-AGCATACATGAGGAAACACACTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K116491-0022-344-B01C-39sm
CTTACATGAGAAGGAGAGGGTTATAATACAGAAATGTCTCATAAAAGAACAGTTCTCTTG
TCTCTACGCCCATTTTCATTACAGATTCCAATCCAACCTGCAAATTTTAAACATTGTTGT
ATATTACCAAGACTGAAAACTCAAACAATGGCTTCAAATCAAAATCTAAGATCATTGTTT
GCAAGAATAAGAGTTCAAATTTTAACCTTTCCACAAACATAAAGACCACCAATATACAGA
GCAACCTGGAGAATCAAGATAGCGAATCTCAAATCAGCTAGTTAGATGATATAAAGCAGG
AAGAAAAATTTGATAGTATGGAACAATCAGCGAGAATCTAAGAACTTTGAGTAAATGAAC
AAAACCTTGAGACTTCGAAGGAATATAGGAGTTTGCAATTTCGCATAAGTACTCAGAAGC
ATACTTGCAGTCTTCAAGTCATTATCTCCTGTCCAACACCACAAACCATCCAGAAAAAAT
GTAATCAAAATCACTAAGCAGAAACTGATTTTTCTAGTAGCCAAGATTTATTATAAAAAC
AAAAACTAACCGAGATTAGCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 11-K116490-0022-344-B01C-8409
GenBank Accession LN491282 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K116490-0022-344-B01C-8409
TCATTGAATATATCCTGGCTAATCTCGGTTAGTTTTTGTTTTTATAATAAATCTTGGCTA
CTAGAAAAATCAGTTTCTGCTTAGTGATTTTGATTACATTTTTTCTGGATGGTTTGTGGT
GTTGGACAGGAGATAATGACTTGAAGACTGCAAGTATGCTTCTGAGTACTTATGCGAAAT
TGCAAACTCCTATATTCCTTCGAAGTCTCAAGGTTTTGTTCATTTACTCAAAGTTCTTAG
ATTCTCGCTGATTGTTCCATACTATCAAATTTTTCTTCCTGCTTTATATCATCTAACTAG
CTGATTTGAGATTCGCTATCTTGATTCTCCAGGTTGCTCTGTATATTGGTGGTCTTTATG
TTTGTGGAAAGGTTAAAATTTGAACTCTTATTCTTGCAAACAATGATCTTAGATTTTGAT
TTGAAGCCATTGTTTGAGTTTTCAGTCTTGGTAATATACAACAATGTTTAAAATTTGCAG
GTTGGATTGGAATCTGTAATGAAAATGGGCGTAGAGACAAGAGAACTGTTCTTTTATGAG
ACATTTCTGTATTATAACCCTCTCCTTCTCATTGTAAGTTACTTTTCTGCTCTCAAATTA
GTAAAGTCAAATTGCTTCAAGTGTGTTTCCCAAGGTATG

Last Updated on 06.12.2018 16:11