Sequences from confirmation amplicon


Confirmation sequences for line 344D04 and hit At5g20250 are shown.
Line ID 344D04
Locus At5g20250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6834932
Primers used for PCR NK07 - 8474, show primer details
   
Sequence Name 36-K102592-0022-344-D04C-8409
GenBank Accession LN502178 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K102592-0022-344-D04C-8409
GGAATGAGCATTTACAATTGATAAAACCAGCTGAGAGACGACGGTTGGCAATCAGTTGAG
AGAGATGCCACGGTGGAGGCCGGAGATGAGAAGAAAGAGTCACCGATTTTTCGACTGACG
GGGATCAAGGAGAACGAAAAGTTTAAGAAGAAGGATGATCCAAACGTTGGGATTAAGAAC
ATTGTCAAGATTGCTAAGGAGAAACACGGTTTGAAATATGTTTACGTGTGGCACGCGATA
ACGGGTTATTGGGGCGGAGTTAGACCCGGGGAAGAATACGGATCTGTTATGAAATATCCT
AATATGTCAAAGGGTGTGGTGGAGAATGATCCGACGTGGAAGACTGATGTAATGACGCTG
CAAGGGTTGGGTTTGGTTAGCCCCAAGAAAGTGTATAAGTTTTACAATGAGCTTCATAGT
TACTTGGCTGATGCTGGCGTGGACGGTGTGAAAGTGGATGTGCAGT
   
Sequence Name 36-K102593-0022-344-D04C-NK07
GenBank Accession LN502179 [GenBank]
Sequencing primer gene specific primer, NK07: 5'-AGTCTCCAATACACACTGCACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K102593-0022-344-D04C-NK07
CTATGAAGCTCATTGTAAAACTTATACACTTTCTTGGGGCTAACCAAACCCAACCCTTGC
AGCGTCATTACATCAGTCTTCCACGTCGGATCATTCTCCACCACACCCTTTGACATATTA
GGATATTTCATAACAGATCCGTATTCTTCCCCGGGTCTAACTCCGCCCCAATAACCCGTT
ATCGCGTGCCACACGTAAACATATTTCAAACCGTGTTTCTCCTTAGCAATCTTGACAATG
TTCTTAATCCCAACGTTTGGATCATCCTTCTTCTTAAACTTTTCGTTCTCCTTGATCCCC
GTCAGTCGAAAAATCGGTGACTCTTTCTTCTCATCTCCGGCCTCCACCGTGGCATCTCTC
TCAACTGATTGCCAACCGTCGTCTCTCAGCTGGTTTTATCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11