Sequences from confirmation amplicon


Confirmation sequences for line 344E11 and hit At2g22460 are shown.
Line ID 344E11
Locus At2g22460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9533367
Primers used for PCR 48ra - 8474, show primer details
   
Sequence Name 29-K115914-0022-344-E11C-48ra
GenBank Accession LN499433 [GenBank]
Sequencing primer gene specific primer, 48ra: 5'-GCCGAATTATTCTCATTTTGTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K115914-0022-344-E11C-48ra
CAACAAGAATAAAAAAATTAAAACTCCAAATCCAAGGGACTATCGAAACGGTATGTAAAT
TACATACAAAGCCAGAGCTAAAACCAAAAGCTTTAGCACGACTCAAAAACAAGTTCTTTT
CTCTAGACCCAAGGAAAAAATAAAAGGAAAGAAACTTGAAAACAGAACTCTTATGTACGA
GCATCTCCACGGGTAATCAAATCAGACTGATGATGGGACAATGAAGATAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 03-K115913-0022-344-E11C-8409
GenBank Accession LN487628 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115913-0022-344-E11C-8409
CATCATCAGTCTGATTTGATACCCGTGGAGATGCTCGTACATAAGAGTTCTGTTTTCAAG
TTTCTTTCCTTTTATTTTTTCCTTGGGTCTAGAGAAAAGAACTTGTTTTTGAGTCGTGCT
AAAGCTTTTGGTTTTAGCTCTGGCTTTGTATGTAATTTACATACCATTTCGATAGTCCAT
TGATTTGAGTTTTAATTTTTTTATCTTGTTGCTATCATTTCTGTACTACATAAATAATGG
TCTTGTCTTTAAAAAAACTGATTATTTAGAGACAAAATGAATTTAATTCGG

Last Updated on 06.12.2018 16:11