Sequences from confirmation amplicon


Confirmation sequences for line 344G05 and hit At4g19040 are shown.
Line ID 344G05
Locus At4g19040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10432458
Primers used for PCR 13ki - 8474, show primer details
   
Sequence Name 54-K113393-0022-344-G05C-8409
GenBank Accession LN508541 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K113393-0022-344-G05C-8409
CATTTCATTGAATATAACACCATATGGATCAGCAATGAAATTCCCGAAATAAAACACCAT
ACTGTAGTGTGTTGATCCTGGAACCTATATAAACACCAGTATTAAACCTATATAAATATG
ACTTTGGTTTGTACTCAATGTTAGGAATCTTAATGACAGTTGATGGGCTAAGCTTACTTG
AACATTTACCACCATTGAGAATAGACCTTTTTCTGCAGCAACCTGAACATGTGTATCAAA
AGACTCAATAATCTTTCTAAGTGATTAAGATTCATCCACAAGCTAAGGAATAGCATGGGA
AGAATATTGAGTCATTGAGTGGAATATTCTCTCCATTAGGTAACTAAAATTAAGGTGCTT
GTGTATCTTACTTGTGCTGCGCAGCCTTTGCGTCTAGCAACATGATCTATTCTTTTACTG
TCTTTGAACCAGTCAACAGCAACGAGATCCATAAGATGCTTGCCAGCAGGTATCTTCCGT
ATAGTGTTAAATGGACAGAAACAAAAGTTAGTTGGCTGTAAGAACCTAACCATTCTATAT
TAATAAGATGAAAATTTTGCCAAGCTTTCTTTTCTCTT
   
Sequence Name 73-K113394-0022-344-G05C-13ki
GenBank Accession LN512936 [GenBank]
Sequencing primer gene specific primer, 13ki: 5'-GAATTTCGGTCAAGAGAAAAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K113394-0022-344-G05C-13ki
GTTAGGTTCTTACAGCCACTAACTTTTGTTTCTGTCCATTTAACACTATACGGAAGATAC
CTGCTGGCAAGCATCTTATGGATCTCGTTGCTGTTGACTGGTTCAAAGACAGTAAAAGAA
TAGATCATGTTGCTAGACGCAAAGGCTGCGCAGCACAAGTAAGATACACAAGCACCTTAA
TTTTAGTTACCTAATGGAGAGAATATTCCACTCAATGACTCAATATTCTTCCCATGCTAT
TCCTTAGCTTGTGGATGAATCTTAATCACTTAGAAAGATTATTGAGTCTTTTGATACACA
TGTTCAGGTTGCTGCAGAAAAAGGTCTATTCTCAATGGTGGTAAATGTTCAAGTAAGCTT
AGCCCATCAACTGTCATTAAGATTCCTAACATTGAGTACAAACCAAAGTCATATTTATAT
AGGTTTAATACTGGTGTTTATATAGGTTCCAGGATCAACACACTACAGTATGGTGTTTTA
TTTCGGGAATTTCATTGCTGATCCATATGGTGTTTATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11