Genotyping and primer details


Genotyping and primer details for 344G05 - At4g19040
Line ID 344G05
Locus At4g19040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10432458 of line 344G05 Chr4:10432458
   
Insertion allele amplicon 8474 - 13ki
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13ki: 5'-GAATTTCGGTCAAGAGAAAAGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10433002
   
wt amplicon (over InsPos) 10ks - 13ki
wt amplicon start Chr4:10432064
wt amplicon end Chr4:10433002
wt amplicon size 939 bp
Locus specific primer 13ki: 5'-GAATTTCGGTCAAGAGAAAAGAAA-3'
2nd locus specific primer 10ks: 5'-CAACGGTTGATGAACCAATATCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11