Sequences from confirmation amplicon


Confirmation sequences for line 344H04 and hit At4g01700 are shown.
Line ID 344H04
Locus At4g01700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:732459
Primers used for PCR BD63 - 8474, show primer details
   
Sequence Name 12-K028344-022-344-H04C-8409
GenBank Accession LN491432 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K028344-022-344-H04C-8409
ATTGAATATATCCTGCGAGAACAAAGACCCTTCTCCTAAATATAATTAATTTTGATCCAC
AGGACCTAATTTAGACTGCGAGAACCAAAGACCCTTCTCCTAAATATAACTATAACTCAA
ATCTATGTATCAAAATGAAATTGAAGTTTGATATCTCAAAATATTTAATAAAACTCACGC
AAGCTGGGAGATTTAATGTTTTACATTTACTTGTAAGCGTACCGTGACATGAAGTTGTTG
ACCTCAATCGCGGTTTCATATGCGCCTTCTACACACCGGCCTAAGGCTACACCAGCGACG
TAATTGCCACCCAAAAATAGCCCTTCGTAGCCCGAAGACGTTAGAGATGATTTAGCCGTG
TCAAGGATATCAAAGTGACCAACTAGAAACTGAGGAATGGCTTGAGGCCATACCCTAACT
CCTAATTTAAGTGGATCGGTCGAATTAGGCTTAATTAGCATTTTCCTCAAATCTCTGTCA
ACTGCTTCCACTAACTCACCTTCAGACTGCGGAATCAAAACAAAAGAGAGAGAGAGAAGA
AAACTATCAGTTCTTAGGTTTACTTGGTTGAATAAAGATGTGTTACAAGTGTTTGCTGTT
TTTTACCTTGGACAGAATTCCGGTGTTTGTAGACCCGCCAATGTAGTTCAACAGCAAAAT
TCTTCCGGGCGGTGCGCGATTTGGAAAGAGTGAGGAGCTGTAGATAGTTCCTGAAGAAAT
ACGAGCAAC
   
Sequence Name 12-K028344-022-344-H04C-BD63
GenBank Accession LN491433 [GenBank]
Sequencing primer gene specific primer, BD63: 5'-TTCATAGAATCTTCATGCTGTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K028344-022-344-H04C-BD63
AGCTCCTCACTCTTTCAAATCGCGCACCGCCCGGAAGAATTTTGCTGTTGAACTACATTG
GCGGGTCTACAAACACCGGAATTCTGTCCAAGGTAAAAAACAGCAAACACTTGTAACACA
TCTTTATTCAACCAAGTAAACCTAAGAACTGATAGTTTTCTTCTCTCTCTCTCTTTTGTT
TTGATTCCGCAGTCTGAAGGTGAGTTAGTGGAAGCAGTTGACAGAGATTTGAGGAAAATG
CTAATTAAGCCTAATTCGACCGATCCACTTAAATTAGGAGTTAGGGTATGGCCTCAAGCC
ATTCCTCAGTTTCTAGTTGGTCACTTTGATATCCTTGACACGGCTAAATCATCTCTAACG
TCTTCGGGCTACGAAGGGCTATTTTTGGGTGGCAATTACGTCGCTGGTGTAGCCTTAGGC
CGGTGTGTAGAAGGCGCATATGAAACCGCGATTGAGGTCAACAACTTCATGTCACGGTAC
GCTTACAAGTAAATGTAAAACATTAAATCTCCCAGCTTGCGTGAGTTTTATTAAATATTT
TGAGATATCAAACTTCAATTTCATTTTGATACATAGATTTGAGTTATAGTTATATTTAGG
AGAAGGGTCTTTGGTTCTCGCAGTCTAAATTAGGTCCTGTGGATCAAAATTAATTATATT
TAGGAGAAGGGTCTTTGGTTCTCGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATT

Last Updated on 06.12.2018 16:11