Sequences from confirmation amplicon


Confirmation sequences for line 345A08 and hit At5g40645 are shown.
Line ID 345A08
Locus At5g40645 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16280412
Primers used for PCR 25rq - 8474, show primer details
   
Sequence Name 49-K116204-0022-345-A08C-25rq
GenBank Accession LN506952 [GenBank]
Sequencing primer gene specific primer, 25rq: 5'-AGCTCCATTGTTGAATCTGAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K116204-0022-345-A08C-25rq
GAACTTGATGCCTTCGCTCGCAGGTAAAAAGGCAAAAAAAAAACACACCCATTCTTGTTA
CGGCTTTCTACTTCGGATTCTAAAATGTGTTTTTGGTTTTGGTTAGGCTTCAGGGATTAA
CCAAATCAGTATCAAAATATCCAACATTCAAAAAACATTTTGTGGAACTAGTCAAAAAAC
TATCAAATGATCTAAACAATAAACATAACCGTTTTGAAGGCGGTTCAAGATCATTTCCAC
GTCCCGGGAAAACGGTGTCTCGAATTTTCACCCAAAAATCGTTCAAAAAAAAAACAAGTA
CCATTGGGTCAAATCAAAATTCACCAAACAAAGGGTTAAAAAACATTGATTTTGTGTAAG
TAAATGTTATGCAACCAAAATTAAACCAAAACTTCGTCGGCTTTTACCTCTGTAAGGATA
AATTTATACAAATCCCAAGTGAATATAAAACTGTTTCATTTATCTAACAAATGTTTTACA
TTCACCTTTTGCTTGATAATGTGTTGTTTCTCAATTGTCCCAAATGTTTCGTCTGCTTCG
TCGGCTTTTGCTTTCATTTCAGGATATATCCAATTGTAAATGGCTTCATGTCCGGAAAAT
CTACTTGGATCACCATTGAGTATAATGGTCAATATGGAAAAAAAAAAAAAGTATTTACCA
ATTTTTTTTCATTTCAAAAATGTAAA
   
Sequence Name 13-K116203-0022-345-A08C-8409
GenBank Accession LN492199 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K116203-0022-345-A08C-8409
ATTTACATTGAATATATCCTGAAATGAAAGCAAAAGCAGACGAAGCAGACGAAACATTTG
GGACAATTGAGAAACAACACATCATCAAGCAAAAGCTGAATGTAAAACATTTGTTAGATA
AATGAAACAGTTTCATATTCACTTGGAATCTGTATAAATTTATCATTACAGAGCTAAAAG
CAGACGAAGTTTCGGTCTAATTCTGGTCGCATAACATTTACTTACACAATATCAATGTCT
CTTAACCCTCTGTTTGGTGAATCTTGATCTGACCCATTGCTACTTGTCTTTTTCTTGAAC
GATTTTTGGCTGAAAATCCGAGACACCGTTTTCCCGGGACGTGGAACTGATCTCGAACCG
CCTTCAAAACGGTTATGTTTATTGTCTAGATCATTTGATAGTTTCTTGACTAGTTCCACA
AAATGTCTTCTGAATGTTGGATATCTTGATACTGATTTGGTTAATCCTTGAAGCCTAACC
AAAACCAAAAACACATCTTAGAATCCGAAGTAGAAAGCCGTAACAAGATTGTGTGTGTTT
TTTTTTTTGCCTTTTTTACCTGCAAGCAAAGGCTTCAAGTTCGGCTTTTCTAAGTTCATT
TAGAGGAGGAAATTCAAATTCA

Last Updated on 06.12.2018 16:11