Sequences from confirmation amplicon


Confirmation sequences for line 345B11 and hit At5g46240 are shown.
Line ID 345B11
Locus At5g46240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18745362
Primers used for PCR 22kh - 8474, show primer details
   
Sequence Name 74-K113549-0022-345-B11C-22kh
GenBank Accession LN513111 [GenBank]
Sequencing primer gene specific primer, 22kh: 5'-AGCCTCTTGTTTAACTACAACGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K113549-0022-345-B11C-22kh
TGGCGTCTCCGGCGAGTTAGCTCGCTATTTGCAAGGTTTGCTTCTATGCTAACTAAGAGA
CCATACTCTTCTTAGGGTTTAACTAAGAAAGTGCTTACTCAAGTCTAACTAACTCTTACC
TTTGCTTCAGGCTTGAGAAAGATATCCGTTTCAACTATTTCTGGATACGTTGCACAAAAC
TCATTTCGGTTAGTTCCAAAACAGAGCAAAACTAATCTCACTTATAATAAAGCATTTTAA
GCTTAGGGCTGTCCATTTAGGTGATCTATTCTTAGGGTTTATTTGTCTTTTAAATGGACA
CCATATTAAAGCTAGTGACTTACACGTGCTTGTTGATCTCAGGTCACTTTGTTCGCTATA
CATTGTGCTGGATGTTTCAACTACCTGATTGCAGATAGATATCCTAATCCAAGAAAGACA
TGGATTGGAGCTGTGTATCCAAATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCACGTTTATTATAAGTGAGGT
   
Sequence Name 09-K113548w-0022-345-B11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K113548w-0022-345-B11C-8409
CATTTACATTGAATTTGGATACACAGCTCCATCCATGTCTTTCTTGGATTAGGATATCTA
TCTGCAATCAGGTAGTTGAAACATCCAGCACAATGTATAGCGAACAAAGTGACCTGAGAT
CAACAAGCACGTGTAAGTCACTAGCTTTAATATGGTGTCCATTTAAAAGACAAATAAACC
CTAAGAATAGATCACCTAAATGGACAGCCCTAAGCTTAAAATGCTTTATTATAAGTGAGA
TTAGTTTTGCTCTGTTTTGGAACTAACCGAAATGAGTTTTGTGCAACGTATCCAGAAATA
GTTGAAACGGATATCTTTCTCAAGCCTGAAGCAAAGGTAAGAGTTAGTTAGACTTGAGTA
AGCACTTTCTTAGTTAAACCCTAAGAAGAGTATGGTCTCTTAGTTAGCATAGAAGCAAAC
CTTGCAAATAGCGAGCTAACTCGCCGGAGACGCCATAACCTGAGCATGCTAAGAATTCTG
AATCCTAGTTCGCTTCCGTTGTAGTTA

Last Updated on 06.12.2018 16:11