Sequences from confirmation amplicon


Confirmation sequences for line 345D05 and hit At5g62880 are shown.
Line ID 345D05
Locus At5g62880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25238250
Primers used for PCR KP11 - 8474, show primer details
   
Sequence Name 07-K025673-022-345-D05C-8409
GenBank Accession LN489106 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K025673-022-345-D05C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACTCAATAAGAACAG
TCATATAACATTAGAAATGACTCATTCTGACACTATAAGAGTCTTATCGTTTTCAACAAT
TAGAGAAAAGGATCATTTATTCAATCAGACCTTTTTAAAAACATTCTCGTAGCTAGCTCG
GCTGACTAATGAGAAAGACAAGACGAAAACATCTGCTCCCCTGTAACTTAAAGGCCTTAA
TCTGTTATAGTCTTCTTGCCCTGCAAACAAAGGATATACATATGTAACCTCATATGAGAC
AAAGCTATTCAATGGATCTCATTTATCAACCAAATGAAAGAACTAGACCAATAAGAATGT
TATAAACCACTATAGGATTCAAGACTCAGAAAGAGTGTTCATACCAGCAGTGTCCCAAAG
CCCCAAATTGACAGTGGTGCCTTCAACAACAACATTTGCACTAAAGTTGTCAAAAACTGT
TGGTATGTAGTCCTGTTACAAACAATTCATCAAATCAATACACAGTTTCAAACTTTCAAT
CATCAAATCAAGCATATATACATATAATAATAATAATAATAGCAATGAAGAAAAA
   
Sequence Name 08-K025673-022-345-D05C-KP11
GenBank Accession LN489570 [GenBank]
Sequencing primer gene specific primer, KP11: 5'-CTGGATTCGTCAGACTTTTTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K025673-022-345-D05C-KP11
ATTATTATTATTATTATATGTATATATGCTTGATTTGATGATTGAAAGTTTGAAACTGTG
TATTGATTTGATGAATTGTTTGTAACAGGACTACATACCAACAGTTTTTGACAACTTTAG
TGCAAATGTTGTTGTTGAAGGCACCACTGTCAATTTGGGGCTTTGGGACACTGCTGGTAT
GAACACTCTTTCTGAGTCTTGAATCCTATAGTGGTTTATAACATTCTTATTGGTCTAGTT
CTTTCATTTGGTTGATAAATGAGATCCATTGAATAGCTTTGTCTCATATGAGGTTACATA
TGTATATCCTTTGTTTGCAGGGCAAGAAGACTATAACAGATTAAGGCCTTTAAGTTACAG
GGGAGCAGATGTTTTCGTCTTGTCTTTCTCATTAGTCAGCCGAGCTAGCTACGAGAATGT
TTTTAAAAAGGTCTGATTGAATAAATGATCCTTTTCTCTAATTGTTGAAAACGATAAGAC
TCTTATAGTGTCAGAATGAGTCATTTCTAATGTTATATGACTGTTCTTATTGAGTCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT

Last Updated on 06.12.2018 16:11