Sequences from confirmation amplicon


Confirmation sequences for line 345D11 and hit At2g21050 are shown.
Line ID 345D11
Locus At2g21050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9035527
Primers used for PCR TK07 - 8474, show primer details
   
Sequence Name 11-K103674-0022-345-D11C-TK07
GenBank Accession LN491079 [GenBank]
Sequencing primer gene specific primer, TK07: 5'-ATGTAGACAGTGAAGCTGACGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K103674-0022-345-D11C-TK07
GGAATATGATANCAGAAACCAAATGGGTATAACGACAGGGAGCCTCGCAGCGGCTCGTTT
ACACATGCTTCTGCACTCATGCATCCCTATTAGCTTCTCCCACACAAAGTAGAGTGGCGT
GCAAGCAAAACCAAAGGTGATGAACTGATGGATGAGCATAAGCACAACTGCAAAGTCACG
GTAAAGATTCTTTGGGAGAAGAGCAAATGCGTTTGAATGGTTTAGAAGCAAATCACCAAA
CGCCCAATAAACCGCAGACGCAGAAGGCAGCGTTAGCGTCAGCACGTAGAGTGTTGCAAA
CAGGTATATGGACTTGAACTTCTGAGGTTTCCACATAGCATGCATTATCTCTCTGCCAAC
AAACAACACACAAACAATGAAAGTGAGTGGTCATTTTCATTACTCTGTAATTCCAGTTAT
ACCCTTAGGATATGTGAATGGTGATGGGGTTAAAAGGGTAAAAAAAAGAGAACATACACA
GTAACAGCATGTCCACCGAATGTGTAAAGAATGTTTGTGGCCCCTGTGAAGTATAAAACC
AGCTTGCTTGGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
   
Sequence Name 27-K103675-0022-345-D11C-8409
GenBank Accession LN498351 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K103675-0022-345-D11C-8409
TTACAATTGACCAAGCAAGCTGGTTTTATACTTCACAGGGGCCACAAACATTCTTTACAC
ATTCGGTGGACATGCTGTTACTGTGTATGTTCTCTTTTTTTTACCCTTTTAACCCCATCA
CCATTCACATATCCTAAGGGTATAACTGGAATTACAGAGTAATGAAAATGACCACTCACT
TTCATTGTTTGTGTGTTGTTTGTTGGCAGAGAGATAATGCATGCTATGTGGAAACCTCAG
AAGTTCAAGTCCATATACCTGTTTGCAACACTCTACGTGCTGACGCTAACGCTGCCTTCT
GCGTCTGCGGTTTATTGGGCGTTTGGTGATTTGCTTCTAAACCATTCAAACGCATTTGCT
CTTCTCCCAAAGAATCTTTACCGTGACTTTGCAGTTGTGCTTATGCTCATCCATCAGTTC
ATCACCTTTGGTTTTGCTTGCACGCCACTCTACTTTGTGTGGGAGAAGCTAATAGGGATG
CATGAGTGCAGAAGCATGTGTAAACGAGCCGCTGCGAGGCTCCCTGTCGTTATACCCATT
TGGTTTCTTGCTATCATATTCCCGTTCTTTGGTCCCATTAACTCAACCGTAGGATCTCTT
CTCGTCAGCTTCACTGTCT

Last Updated on 06.12.2018 16:11