Sequences from confirmation amplicon


Confirmation sequences for line 345E07 and hit At2g27670 are shown.
Line ID 345E07
Locus At2g27670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11801571
Primers used for PCR GD59 - 8474, show primer details
   
Sequence Name 83-K033712-022-345-E07C-GD59
GenBank Accession LN514259 [GenBank]
Sequencing primer gene specific primer, GD59: 5'-GGACAACAAATCAACAAAGGTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K033712-022-345-E07C-GD59
AAAAACTTTGAGATATAAGCTCCTTGGGCGCTCTATAGCTATCCCGATACATCTAATCAT
CGATGAAAACCATAAAAACCTAACGGTAAGAAGCTTCTCCAAACCTTTCACATGAACCAA
TTGTTAAATTTTGTGTTTTGGAGGAAGTGAATATTTTGGTTGATCTATTGCTTAGGCAAA
ATATAAGAAAGAGAAAATGATGTACATGAAAGTGTTTGAGGGTAGATGGAAAGTGGAGCC
ACTATACGCAGATCAGGAACGCTTATGCAAGTCTAGGTCGCGGATTAATGAAGAAGAGTA
CAAAAAATGTAGTGACGGAAAAGGAAGGATTGGATCAAAGGTGACAATGGAGCAGATCTT
TCAGCCTTGTTCTCTTCTTAATGTGCCACCGATTTCTTGGTTCATCCGTGAGATCACCAT
CAAAACAATAAAGAATTTGCTCGAAGATCTTAGACAATTTGTTATCGACATGCACAAAAA
TTCAGACCCGGAAAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTCAC
   
Sequence Name 67-K033712-022-345-E07C-8409
GenBank Accession LN511746 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K033712-022-345-E07C-8409
TTACATTGAAATATTTTCCGGGTCTGAATTTTTGTGCATGTCGATAACAAATTGTCTAAG
ATCTTCGAGCAAATTCTTTATTGTTTTGATGGTGATCTCACGGATGAACCAAGAAATCGG
TGGCACATTAAGAAGAGAACAAGGCTGAAAGATCTGCTCCATTGTCACCTTTGATCCAAT
CCTTCCTTTTCCGTCACTACATTTTTTGTACTCTTCTTCATTAATCCGCGACCTAGACTT
GCATAAGCGTTCCTGATCTGCGTATAGTGGCTCCACTTTCCATCTACCCTCAAACACTTT
CATGTACATCATTTTCTCTTTCTTATATTTTGCCTAAGCAATAGATCAACCAAAATATTC
ACTTCCTCCAAAACACAAAATTTAACAATTGGTTCATGTGAAAGGTTTGGAGAAGCTTCT
TACCGTTAGGTTTTTATGGTTTTCATCGATGATTAGATGTATCGGGATAGCTATAGAGCG
CCCAAGGAGCTTATATCTCAAAGTTTTTTTCACTCTCGTGATCTGCCGCGGTCCGTCCTT
CTTCAAAACCTTTGTTGANTTGTTGTC

Last Updated on 06.12.2018 16:11