Sequences from confirmation amplicon


Confirmation sequences for line 345H07 and hit At1g50580 are shown.
Line ID 345H07
Locus At1g50580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18731027
Primers used for PCR KH45 - 8474, show primer details
   
Sequence Name 34-K103228-0022-345-H07C-KH45
GenBank Accession LN501376 [GenBank]
Sequencing primer gene specific primer, KH45: 5'-GCATAGCTTACCTTCAAGTTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K103228-0022-345-H07C-KH45
AGTTCTTAGGCCTTTGGTGATCAGACCGAAAAGCTCATGGGAATTCGCAAAGAGAGAACA
GACGTTAGCTTCATGTCCACGTAACGCCACTTTGGATAAAGGATAATCCGGCGGAGGAAA
CCCTAATTCAGCCCTAGGTGCAAGAACCATAGCTACACAAGCTGCCGATATGATCTGGTA
ATTGACACTCTTTATTCCAAACTCTTCTGCCATTTCTGGAACCCAATGAACAAAATCGAA
AAAGATTAGATCTGGTTTCAAAGCACGGACCTTTGCTTCGATCTGATCGCGTAAGAGATC
CATGGCAACGAANATGGGTTTCTTAGTTGAGTTTGGGAGATCCGAGGCTGTCTCGGCGCC
NAAAGGGAGACCATCGACAGCAATATTCTTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 02-K103229-0022-345-H07C-8409
GenBank Accession LN486990 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K103229-0022-345-H07C-8409
TTCGGACATGAGCCTTTACATTGAAGAATATTGCTGTCGATGGTCTCCCTTTTGGCGCCG
AGACAGCCTCGGATCTCCCAAACTCAACTAAGAAACCCATATTCGTTGCCATGGATCTCT
TACGCGATCAGATCGAAGCAAAGGTCCGTGCTTTGAAACCAGATCTAATCTTTTTCGATT
TTGTTCATTGGGTTCCAGAAATGGCAGAAGAGTTTGGAATAAAGAGTGTCAATTACCAGA
TCATATCGGCAGCTTGTGTAGCTATGGTTCTTGCACCTAGGGCTGAATTAGGGTTTCCTC
CGCCGGATTATCCTTTATCCAAAGTGGCGTTACGTGGACATGAAGCTAACGTCTGTTCTC
TCTTTGCGAATTCCCATGAGCTTTTCGGTCTGATCACCAAAGGCCTTAAGAACTGTGACG
TCNTTTCCATAAGGACCTGCGTGGAANTTGAAGGTAAGCTAT

Last Updated on 06.12.2018 16:11