Sequences from confirmation amplicon


Confirmation sequences for line 346A05 and hit At1g01470 are shown.
Line ID 346A05
Locus At1g01470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:172373
Primers used for PCR 19qz - 8474, show primer details
   
Sequence Name 68-K115869-0022-346-A05C-19qz
GenBank Accession LN512076 [GenBank]
Sequencing primer gene specific primer, 19qz: 5'-GCAGAATTAAAATGGAAACCGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K115869-0022-346-A05C-19qz
CCAAATTGAATTATAAGATTTTGCCTTGGATTTTGGTTTGGTTTCTTATGCAACTTCCCA
AAAGAACTGATCTGTCCAAACTTATGATATGGCAAAATAACATTTTATTAGTTATAGCAA
ACCCAACTTATTACATTACGATCATCACCATTCACCATCATAACGATGTGTGGTCTTATA
TATAGGTTTTGTCGATCAGAAGAAATCTTTAAAAGTAGGAAGTTTGATTTCTCCCTTACT
AGAGATAGGGATAGTAAATTCCCCTACAACAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 27-K115867-0022-346-A05C-8409
GenBank Accession LN498538 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115867-0022-346-A05C-8409
ATTACATTGAATATATCCTGTTGTAGGGGAATTTACTATCCCTATCTCTAGTAAGGGAGA
AATCAAACTTCCTACTTTTAAAGATTTCTTCTGATCGACAAAACCTATATATAAGACCAC
ACATCGTTATGATGGTGAATGGTGATGATCGTAATGTAATAAGTTGGGTTTGCTATAACT
AATAAAATGTTATTTTGCCATATCATAAGTTTGGACAGATCAGTTCTTTTGGGAAGTTGC
ATAAGAAACCAAACCAAAATCCAAGGCAAAATCTTATAATTCAATTTGGTTTGATATGAA
AACCAAAAGATATGTTCTGTTTTGTTCTGATTTATCGGTTTCCATTTAAATTCTG

Last Updated on 06.12.2018 16:11