Sequences from confirmation amplicon


Confirmation sequences for line 346C01 and hit At1g60350 are shown.
Line ID 346C01
Locus At1g60350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22241419
Primers used for PCR 12oh - 8474, show primer details
   
Sequence Name 23-K114963-0022-346-C01C-12oh
GenBank Accession LN496669 [GenBank]
Sequencing primer gene specific primer, 12oh: 5'-TTCTCTCTTAAGTCTCTGGCAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K114963-0022-346-C01C-12oh
ATCACACACACAATCTCTGCTTAGCAGATAAAAAAACATGTCAATTTAATTGATCTTCAT
TTAATTTATAGATTGTTCGGGTTGCTGATTCAGAAACATGTAGGTTGTTGTCGAATCATA
TGTACATGTTTGGTTACATAAGGCTCAATTTGTCTCTGATGTTTGTGATTGCTGCTAATA
TCGATTGTTTACATGCGATTAGTGTTATTTTGGGTTTAAAATATTGCAGCGACAAAAAAG
CCTCTCCTCTCACAATCACAAACCACATGAAAGAGAGAAAAAAGCACACTAGTCTCTAAA
TCATATTGAACAGAGAAATATAAACACAAAGACTATCGATATATGTTTTAAAGAGAATGT
CCTCAAGATACTAAGATGATCATAAAATCCAAAGAAAAGATTCTTAGTTATCATAAGTAA
ACTTGATAACGCACAAGAATTTATTCTGCTTCACGCTAAGCCTATACTCTTCTAGAGTCC
AAGTTACCTTCACATCTTCTCCACCACAGAAGTATCTTTCTTTCTCCGTTTCATGAAACT
TAAACACCTTATATAGAGGACCACCAGCCTACATGATCATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCG
   
Sequence Name 07-K114962-0022-346-C01C-8409
GenBank Accession LN489393 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K114962-0022-346-C01C-8409
TATGATCATGTAGGCTGGTGGTCCTCTATATAAGGTGTTTAAGTTTCATGAAACGGAGAA
AGAAAGATACTTCTGTGGTGGAGAAGATGTGAAGGTAACTTGGACTCTAGAAGAGTATAG
GCTTAGCGTGAAGCAGAATAAATTCTTGTGCGTTATCAAGTTTACTTATGATAACTAAGA
ATCTTTTCTTTGGATTTTATGATCATCTTAGTATCTTGAGGACATTCTCTTTAAAACATA
TATCGATAGTCTTTGTGTTTATATTTCTCTGTTCAATATGATTTAGAGACTAGTGTGCTT
TTTTCTCTCTTTCATGTGGTTTGTGATTGTGAGAGGAGAGGCTTTTTTGTCGCTGCAATA
TTTTAAACCCAAAATAACACTAATCGCATGTAAACAATCGATATTAGCAGCAATCACAAA
CATCAGAGACAAATTGAGCCTTATGTAACCAAACATGTACATATGATTCGACAACAACCT
ACATGTTTCTGAATCAGCAACCCGAACAATCTATAAATTAAATGAAGATCAATTAAATTG
ACATGTTTTTTTATCTGCTAAGCAGAGATTGTTGTTGTGTTGATTTCTCGTGTATATGCA
CATGTAGCCTTTTTGGTTGCCAGAGACT

Last Updated on 06.12.2018 16:11