Sequences from confirmation amplicon


Confirmation sequences for line 346F05 and hit At1g74210 are shown.
Line ID 346F05
Locus At1g74210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27911226
Primers used for PCR PD05 - 8474, show primer details
   
Sequence Name 27-K025267-022-346-F05C-8409
GenBank Accession LN498220 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K025267-022-346-F05C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACGAAACAACC
AATGTTGCGAGCCACAAGGAGTTTGCAGATCGTAAAAGGACATATGATGTCCAAGGATTC
AACATTACTGGCTTTTTCACTTGTACGTTACATCAATTCTTCTTCTGTTTCTAGGTCTGA
GTCAATGCATGCTGCTGGATTCGGTCTCAGGATAGAGTCATTTGCTCAAAATATCTCTCG
CACATAATCTCATAACAGCTAGAGAAGCATCTAATAAAGCTTCAAGTTGGTTTGTTTGAG
TCTGAATGATTTGCTAACAAGTGATTCCTCTTTCATTGCTTGTAGTTGATTTTACACTTA
AAGAACTAAAGCAACTACGTATAAAGCAGCGATATGCTTTTCGGGATCAGCAATACAACG
GTTTGCTCGCCTTTCTTTCCTGTATTTTGGCTGTTGGAAGATATAAAGTAAGAACTGCAT
AATACAGGAAATCTTTAACCATGCCCATATCTTGCTTGTATTTCAGGAATGTACCCTATC
ATTACATTTGAGGAATTTCTTACCATTGCTCGGGATGCTCCAAGAGTTGTTGGAATATAT
CCTGAGATTAAAAATCCAGTTTTAATG
   
Sequence Name 28-K025267-022-346-F05C-PD05
GenBank Accession LN498687 [GenBank]
Sequencing primer gene specific primer, PD05: 5'-GACTCACATGCTGGTTCATTAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K025267-022-346-F05C-PD05
GGATTTTTAATCTCAGGATATATTCCAACAACTCTTGGAGCATCCCGAGCAATGGTAAGA
AATTCCTCAAATGTAATGATAGGGTACATTCCTGAAATACAAGCAAGATATGGGCATGGT
TAAAGATTTCCTGTATTATGCAGTTCTTACTTTATATCTTCCAACAGCCAAAATACAGGA
AAGAAAGGCGAGCAAACCGTTGTATTGCTGATCCCGAAAAGCATATCGCTGCTTTATACG
TAGTTGCTTTAGTTCTTTAAGTGTAAAATCAACTACAAGCAATGAAAGAGGAATCACTTG
TTAGCAAATCATTCAGACTCAAACAAACCAACTTGAAGCTTTATTAGATGCTTCTCTAGC
TGTTATGAGATTATGTGCGAGAGATATTTTGAGCAAATGACTCTATCCTGAGACCGAATC
CAGCAGCATGCATTGACTCAGACCTAGAAACAGAAGAAGAATTGATGTAACGTACAAGTG
AAAAAGCCAGTAATGTTGAATCCTTGGACATCATATGTCCTTTTACGATCTGCAAACTCC
TTGTGGCTCGCAACATTGGTTGTTTCGTCAGGATATATTCAATTGTAAATGGCTTCGTGT
CCGGGAAATCTACGTGGATCAGCAATGAGTATGATGGTCAA
Primers used for PCR AP26 - 8474, show primer details
   
Sequence Name 28-K118837-0022-346-F05C-AP26
GenBank Accession LN499061 [GenBank]
Sequencing primer gene specific primer, AP26: 5'-TGATCTTACAGGATGCTTACCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118837-0022-346-F05C-AP26
TGCACCCTCTCCCTGTTAAAGGACCCAAAACTGTCAAGCTTCAACTTCAGACTTCACGTC
CTTATAACATCGCACACAGAGGTTCCAATGGAGAGATCCCAGAAGAAACTACAGCTGCAT
ACTTGGTATACTGTTGATCTTTCTTGATCAATCTCCTTCTCTTTGATGATTTCCGAAGAT
AAGAAGTAAAACATTTTTTTTTTCTTATGATAGAAAGCAATTGAAGAAGGCACAGACTTC
ATAGAAACAGATATCTTATCATCCATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCG
   
Sequence Name 04-K118836-0022-346-F05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118836-0022-346-F05C-8409
TTTTCCTGGCTCGATTTTCGTGGGGGGGGG

Last Updated on 06.12.2018 16:11