Genotyping and primer details


Genotyping and primer details for 346F05 - At1g74210
Line ID 346F05
Locus At1g74210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27911226 of line 346F05 Chr1:27911226
   
Insertion allele amplicon 8474 - AP26
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AP26: 5'-TGATCTTACAGGATGCTTACCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27910855
   
Insertion allele amplicon 8474 - PD05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PD05: 5'-GACTCACATGCTGGTTCATTAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27911820
   
wt amplicon (over InsPos) AP26 - PD05
wt amplicon start Chr1:27910855
wt amplicon end Chr1:27911820
wt amplicon size 966 bp
Locus specific primer PD05: 5'-GACTCACATGCTGGTTCATTAAAA-3'
2nd locus specific primer AP26: 5'-TGATCTTACAGGATGCTTACCCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11