Sequences from confirmation amplicon


Confirmation sequences for line 346F08 and hit At1g27180 are shown.
Line ID 346F08
Locus At1g27180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9443005
Primers used for PCR 07nm - 8474, show primer details
   
Sequence Name 31-K114574-0022-346-F08C-07nm
GenBank Accession LN500267 [GenBank]
Sequencing primer gene specific primer, 07nm: 5'-AACACAGGGTTTATTGGAGTTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114574-0022-346-F08C-07nm
TAAGTGTTTGGTTCTCATTTTTATGACAAGGACGAAAACGAATGGCAAGTTGAACTAGAG
AAGCTGAAAACCCAACAAGACAAACTTCACGGTGTTCTGGCTCTGAGTTTCAAATCTTTA
GACGAAGAAGAAAAGAAGATATTCCTCGACATTGCATGTCTCTTTCTCAAAATGGATATA
ACAAAAGAAGAAGTTGTCGACATACTGAAAGGATGTGGGTTAAACGCCGAGGCTGCTCTC
CGTGTTCTTATACAGAAATCTCTCCTTACAATCTTGACAGACGACACTCTGTGGATGCAT
GATCAGATCAGAGACATGGGTAGGCAGATGGTTCATAAAGAAAGCAGCGATGATCCTGAG
ATGCGGAGTAGACTCTGGGATCGTGGTGAAATAATGAACGTATTGGCTTACATTCTCACG
TATGAGCAATGAACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAAATGTC
   
Sequence Name 28-K114573-0022-346-F08C-8409
GenBank Accession LN498957 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K114573-0022-346-F08C-8409
TTGTTCATTGCTCATACGTGAGAATGTAAGCCAATACGTTCATTATTTCACCACGATCCC
AGAGTCTACTCCGCATCTCAGGATCATCGCTGCTTTCTTTATGAACCATCTGCCTACCCA
TGTCTCTGATCTGATCATGCATCCACAGAGTGTCGTCTGTCAAGATTGTAAGGAGAGATT
TCTGTATAAGAACACGGAGAGCAGCCTCGGCGTTTAACCCACATCCTTTCAGTATGTCGA
CAACTTCTTCTTTTGTTATATCCATTTTGAGAAAGAGACATGCAATGTCGAGGAATATCT
TCTTTTCTTCTTCGTCTAAAGATTTGAAACTCAGAGCCAGAACACCGTGAAGTTTGTCTT
GTTGGGTTTTCAGCTTCTCTAGTTCAACTTGCCATTCGTTTTCGTCCTTGTCATAAAAAT
GAGAACCAAACACTTTAACAGCCAGTGGCAACAGTCCCGTCACCTCGGCAATCTTCTTGG
ACAACTCCAA
Primers used for PCR 06nm - 8474, show primer details
   
Sequence Name 32-K114574-0022-346-F08C-06nm
GenBank Accession LN500684 [GenBank]
Sequencing primer gene specific primer, 06nm: 5'-GCTGGTATGGTGAAGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K114574-0022-346-F08C-06nm
AGTAGCTCTCGTGAACCACAGTATGAGGTCAAGTGCTTGACTGAACCGCAGGCGTTGAAG
CTCTTTAGTTTTTATTCACTACGAAAAGAGAAACCACCAACACAGGGTTTATTGGAGTTG
TCCAAGAAGATTGCCGAGGTGACGGGACTGTTGCCACTGGCTGTTAAAGTGTTTGGTTCT
CATTTTTATGACAAGGACGAAAACGAATGGCAAGTTGAACTAGAGAAGCTGAAAACCCAA
CAAGACAAACTTCACGGTGTTCTGGCTCTGAGTTTCAAATCTTTAGACGAAGAAGAAAAG
AAGATATTCCTCGACATTGCATGTCTCTTTCTCAAAATGGATATAACAAAAGAAGAAGTT
GTCGACATACTGAAAGGATGTGGGTTAAACGCCGAGGCTGCTCTCCGTGTTCTTATACAG
AAATCTCTCCTTACAATCTTGACAGACGACACTCTGTGGATGCATGATCAGATCAGAGAC
ATGGGTAGGCAGATGGTTCATAAAGAAAGCAGCGATGATCCTGAGATGCGGAGTAGACTC
TGGGATCGTGGTGAAATAATGAACGTATTGGCTTACATTCTCACGTATGAGCAATGAACA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CA
   
Sequence Name 29-K114573-0022-346-F08C-oriL
GenBank Accession LN499400 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114573-0022-346-F08C-oriL
TTGTTCATTGCTCATACGTGAGAATGTAAGCCAATACGTTCATTATTTCACCACGATCCC
AGAGTCTACTCCGCATCTCAGGATCATCGCTGCTTTCTTTATGAACCATCTGCCTACCCA
TGTCTCTGATCTGATCATGCATCCACAGAGTGTCGTCTGTCAAGATTGTAAGGAGAGATT
TCTGTATAAGAACACGGAGAGCAGCCTCGGCGTTTAACCCACATCCTTTCAGTATGTCGA
CAACTTCTTCTTTTGTTATATCCATTTTGAGAAAGAGACATGCAATGTCGAGGAATATCT
TCTTTTCTTCTTCGTCTAAAGATTTGAAACTCAGAGCCAGAACACCGTGAAGTTTGTCTT
GTTGGGTTTTCAGCTTCTCTAGTTCAACTTGCCATTCGTTTTCGTCCTTGTCATAAAAAT
GAGAACCAAACACTTTAACAGCCAGTGGCAACAGTCCCGTCACCTCGGCAATCTTCTTGG
ACAACTCCAATAAACCCTGTGTTGGTGGTTTCTCTTTTCGTAGTGAATAAAAACTAAAGA
GCTTCAACGCCTGCGGTTCAGTCAAGCACTTGACCTCATACTGTTGGTTCACTGAGAGCT
TACTCAGAATCTCAGAATCTCTGGTAGTGATGACTATNAGACTTCCTTCACANTACCAG

Last Updated on 06.12.2018 16:11