Sequences from confirmation amplicon


Confirmation sequences for line 346H01 and hit At2g21727 are shown.
Line ID 346H01
Locus At2g21727 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9278637
Primers used for PCR 07vy - 8474, show primer details
   
Sequence Name 50-K118393-0022-346-H01C-07vy
GenBank Accession LN507316 [GenBank]
Sequencing primer gene specific primer, 07vy: 5'-ATATTATGATCCTAGGCCCCCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K118393-0022-346-H01C-07vy
CATTTTGGATTTGCTCAAACATATCAAATTTATACTTTGAAAAGAAAAGAATCATCAAAA
TAAAGTTATTTTATTATTTTTTCTTTGTTTACTAATCTCACAATCATAACGTCAATTATA
TAGAAATTTTCTTAACATGAGTCAAAATAAGACTAACGCTAAATTTTACATTCTAATTCT
AATTTTAAGTGATGTTTCTGAACCAAATAATATGTATTAAGTTTACTAATAGTTTAACAT
ATTAACATTTAACCAGCATATATGAATAGCTTATCGATTAGAAAGTGATTTCTAACAAAG
CCACACAATAGTCCAGATACACTCAAACCCGATAGTGCATAATTAACAGAACTACTATAA
TCACAAACAAAGTGTATGATTGAGCCACATATTAACCTAGATATAGAAAGATAATATACC
ATGGGAAGTTATATATTATTGTTCAAATAATAAAATTCGTTATATTTTTTAACCAGAAAA
TGGTGAAACAACATTAATTATCAGTAGAACACATGTTCCATATTTCATTAGTTTCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATTC
   
Sequence Name 02-K118392-0022-346-H01C-8409
GenBank Accession LN487234 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K118392-0022-346-H01C-8409
TTACATTGAAATATCCTGAAACTAATGAAATATGGAACATGTGTTCTACTGATAATTAAT
GTTGTTTCACCATTTTCTGGTTAAAAAATATAACGAATTTTATTATTTGAACAATAATAT
ATAACTTCCCATGGTATATTATCTTTCTATATCTAGGTTAATATGTGGCTCAATCATACA
CTTTGTTTGTGATTATAGTAGTTCTGTTAATTATGCACTATCGGGTTTGAGTGTATCTGG
ACTATTGTGTGGCTTTGTTAGAAATCACTTTCTAATCGATAAGCTATTCATATATGCTGG
TTAAATGTTAATATGTTAAACTATTAGTAAACTTAATACATATTATTTGGTTCAGAAACA
TCACTTAAAATTAGAATTAGAATGTAAAATTTAGCGTTAGTCTTATTTTGACTCATGTTA
AGAAAATTTCTATATAATTGACGTTATGATTGTGAGATTAGTAAACAAAGAAAAAATAAT
AAAATAACTTTATTTTGATGATTCTTTTCTTTTCAAAGTATAAATTTGATATGTTTGAGC
AAATCCAAAATGATAGTACCAAATAGGAACACATTCCATATGATTATGAAGAAGGGGGCC
T

Last Updated on 06.12.2018 16:11