Sequences from confirmation amplicon


Confirmation sequences for line 346H07 and hit At5g59240 are shown.
Line ID 346H07
Locus At5g59240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23903490
Primers used for PCR 10kn - 8474, show primer details
   
Sequence Name 78-K113669-0022-346-H07C-10kn
GenBank Accession LN513719 [GenBank]
Sequencing primer gene specific primer, 10kn: 5'-GAGCAGCATCAACTTGAACAATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K113669-0022-346-H07C-10kn
GCTATTGTTTGATGCATTGTACGCCACATCCAAGATCCTAGTCTTGCGGGTCACAGCTTC
ACTTCCCCACGAGAAGTTTCCAGTATCGAGCCTCAAAGCTCGCCACTTTACATTCCCACC
ACGCACACGGATCCTCCTCACCGTCTTGTTACTTGACAGCTTAGTGTTAGCTGGTTGTCT
TCCAAGCTCATACCTTTATAAGAGATAACCAAAGTTAGTTTATGTGATCATCAATTTTGC
TTCAACCAGAAGTTATACAAAGTTATCAAGCATGTAGAAACAACCAAAATTAAAGAACAA
GACAAGCTCATGATTCATTTTGATTCCAATCTACTCTATGATTCAATATTTAAGCAGGGG
AAGATTAAAAAGCTTACTTTCGCTTCTTCCTCCACATTTTTTGCTTAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 47-K113666-0022-346-H07C-8409
GenBank Accession LN506255 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K113666-0022-346-H07C-8409
GCAAAAATGTGGAGGAAGAAGCGAAAGTAAGCTTTTTAATCTTCCCCTGCTTAAATATTG
AATCATAGAGTAGATTGGAATCAAAATGAATCATGAGCTTGTCTTGTTCTTTAATTTTGG
TTGTTTCTACATGCTTGATAACTTTGTATAACTTCTGGTTGAAGCAAAATTGATGATCAC
ATAAACTAACTTTGGTTATCTCTTATAAAGGTATGAGCTTGGAAGACAACCAGCTAACAC
TAAGCTGTCAAGTAACAAGACGGTGAGGAGGATCCGTGTGCGTGGTGGGAATGTAAAGTG
GCGAGCTTTGAGGCTCGATACTGGAAACTTCTCGTGGGGAAGTGAAGCTGTGACCCGCAA
GACTAGGATCTTGGATGTGGCGTACAATGCATCAAACAATGAGCTTGTTCGTACACAAAC
TCTTGTGAAGAGTGCTATTGTTCAAGTTGATGCTGCTCA

Last Updated on 06.12.2018 16:11