Sequences from confirmation amplicon


Confirmation sequences for line 347C07 and hit At3g50840 are shown.
Line ID 347C07
Locus At3g50840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18897185
Primers used for PCR 17vc - 8474, show primer details
   
Sequence Name 54-K117671-0022-347-C07C-17vc
GenBank Accession LN508610 [GenBank]
Sequencing primer gene specific primer, 17vc: 5'-ACAAGCATTCTTTCTCTAGCTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K117671-0022-347-C07C-17vc
CAACGAAGCACCTGATTCTCCTTCACCGCTTCCTTCCATCTACCCAAGTCCACCACCNGT
TTTATCACCGNTCTTCCTCCGTCAGTAAAGTCCCAGTGATTACTTGCCGGAGCTGTAGCT
GCTCCAAAAACAGAACTTGTACGACGGCTCGTAGTGGTAAACGCTCGTTTTGTGCCGCGT
GTGTGCATCCTTCAACAGTCAGTTTTCTACAATCCATCACGCTACAAATCTTCTCCTTCT
CTTCCTCCAATATCCATGAATGAGTCTATAAAAACAAAAATCACAACTCAATCATCAGGT
GCTGTGGTGTAGTGGTTATCACGTTTGCCTTACACGCAAAAGGTCTCCAGTTCGACCCTG
GGCAGCACCAAACCATAGACAATTTTATAAATTTTTATAATTAATTACCTTGAAATATAT
ATCGACGGCTCTGTATAGTCCATCATCGTAAACCCGTGCTTGAACCGGAAGCAAAACCGC
CAGATTATAAAACTGTTCCGGTTTAAGATTAGCGTCCGATGCAATTTCACCGAGAACTCC
GTCGATCAATCTCCCAACCACCGTCAATGACGACGACGACACGGCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
Primers used for PCR 40vk - 8474, show primer details
   
Sequence Name 72-K118684-0022-347-C07C-40vk
GenBank Accession LN512808 [GenBank]
Sequencing primer gene specific primer, 40vk: 5'-CCTCCAATATCCATGAATGAGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K118684-0022-347-C07C-40vk
ATCACTCATCATCAGGTGCTGTGGTGTAGTGGTTATCACGTTTGCCTTACACGCAAAAGG
TCTCCAGTTCGACCCTGGGCAGCACCAAACCATAGACAATTTTATAAATTTTTATAATTA
ATTACCTTGAAATATATATCGACGGCTCTGTATAGTCCATCATCGTAAACCCGTGCTTGA
ACCGGAAGCAAAACCGCCAGATTATAAAACTGTTCCGGTTTAAGATTAGCGTCCGATGCA
ATTTCACCGAGAACTCCGTCGATCAATCTCCCAACCACCGTCAATGACGACGACGACACG
GCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCAGCACGTTATT
Primers used for PCR CT18 - 3144, show primer details
   
Sequence Name 25-K119622-0022-347-C07C-CT18
GenBank Accession LN497688 [GenBank]
Sequencing primer gene specific primer, CT18: 5'-TGTGATCTTATCCATGAAGTCGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K119622-0022-347-C07C-CT18
TCTACTTCTTCGCGAAGATACCGTTCCAGGAATCTCGCGTGCATCGTCAGCATCTTCATC
TTCTTCATCTTCTTCCACCACCATAGCTTCCGAGAATCAACAAAGAGAATTATTAGAGAC
GATAACTTCAGATCTTCCATTAACTGCGACGACTACGAGATCTCTCTTCGGTTTGTTACG
AGCCGCGATCATTCTCAACGCCTCCGAGAATTGCAGGAAGTTTCTAGAGAAAAAAATCGG
ATCGAATCTTGAGAAAGCTACGCTTGATGATCTTCTCATTCCAAGTTACTCGTATCTCAA
CGAAACGTTATACGATATTGATTTGGTGGAGAGACAATCTGATCCCCGGGTACCCTGTCC
TCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATT
GTGCGTCATCCCTTACGTCAGTGGAGATATCAATCCAATCCA
   
Sequence Name 01-K119620-0022-347-C07C-3144
GenBank Accession LN486808 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K119620-0022-347-C07C-3144
CGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGG
GATCAGATTGTCTCTCCACCAAATCAATATCGTATAACGTTTCGTTGAGATACGAGTAAC
TTGGAATGAGAAGATCATCAAGCGTAGCTTTCTCAAGATTCGATCCGATTTTTTTCTCTA
GAAACTTCCTGCAATTCTCGGAGGCGTTGAGAATGATCGCGGCTCGTAACAAACCGAAGA
GAGATCTCGTAGTCGTCGCAGTTAATGGAAGATCTGAAGTTATCGTCTCTAATAATTCTC
TTTGTTGATTCTCGGAAGCTATGGTGGTGGAAGAAGAAGAAGAAGATGAAGATGCTGACG
ATGAACGCGAGATTCCTGGAATGTGTTTCTTCGCGTAAGTAAGTAGAGATCTCTCTACAA
TCTCAGGACTCAGGACACTCGACTTCATGGAAAAGATCACAAA
Primers used for PCR 91wr - 3144, show primer details
   
Sequence Name 26-K119622-0022-347-C07C-91wr
GenBank Accession LN498153 [GenBank]
Sequencing primer gene specific primer, 91wr: 5'-CCGAGTATCTAGAAATGACGGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K119622-0022-347-C07C-91wr
AACGAGAATTTCTTTCAGAGTTCGTCTTCCTAACGTACAAGAATCCATCAAAGCGTTGAA
AGCTTGTGAATCAGTCTCGTCTTTAGCTGAATCACTCTGTATAACGGAACAGTGTATAGA
CTCCATCGTCTTCCAAGCTTCCTCAACCGATCCGTCGTCGTTTTACGGTTGGCCAATAAA
CAACGGAGGTATATTCACCGTTGACCGGAAGAAACAGAGCAAAGATTCGAAAACAGAGCT
TTGGTTCGAAGATCTAACGGAGTTGAGTTTTCCAATCTTCAGACGTGTGATCTTATCCAT
GAAGTCGAGTGTCCTGAGTCCTGAGATTGTAGAGAGATCTCTACTTACTTACGCGAAGAA
ACACATTCCAGGAATCTCGCGTTCATCGTCAGCATCTTCATCTTCTTCTTCTTCTTCCAC
CACCATAGCTTCCGAGAATCAACAAAGAGAATTATTAGAGACGATAACTTCAGATCTTCC
ATTAACTGCGACGACTACGAGATCTCTCTTCGGTTTGTTACGAGCCGCGATCATTCTCAA
CGCCTCCGAGAATTGCAGGAAGTTTCTAGAGAAAAAAATCGGATCGAATCTTGAGAAAGC
TACGCTTGATGATCTTCTCATTCCAAGTTACTCGTATCTCAACGAAACGTTATACGATAT
TGATTTGGTGGAGAGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAAC
TTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGT
CAGTGGAG
   
Sequence Name 02-K119620-0022-347-C07C-35St
GenBank Accession LN487262 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K119620-0022-347-C07C-35St
TTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCTCTCCACCAAATCAATATCGTATAACGTTTCGTTGAGATACGAGTAA
CTTGGAATGAGAAGATCATCAAGCGTAGCTTTCTCAAGATTCGATCCGATTTTTTTCTCT
AGAAACTTCCTGCAATTCTCGGAGGCGTTGAGAATGATCGCGGCTCGTAACAAACCGAAG
AGAGATCTCGTAGTCGTCGCAGTTAATGGAAGATCTGAAGTTATCGTCTCTAATAATTCT
CTTTGTTGATTCTCGGAAGCTATGGTGGTGGAAGAAGAAGAAGAAGATGAAGATGCTGAC
GATGAACGCGAGATTCCTGGAATGTGTTTCTTCGCGTAAGTAAGTAGAGATCTCTCTACA
ATCTCAGGACTCAGGACACTCGACTTCATGGATAAGATCACACGTCTGAAGATTGGAAAA
CTCAACTCCGTTAGATCTTCGAACCAAAGCTCTGTTTTCGAATCTTTGCTCTGTTTCTTC
CGGTCAACGGTGAATATACCTCCGTTGTTTATTGGCCAACCGTAAAACGACGACGGATCG
GTTGAGGAAGCTTGGAAGACGATGGAGTCTATACACTGTTCCGTTATACAGAGTGATTCA
GCTAAAGACGAGACTGATTCACAAGCTTTCAACGCTTTGATGGATTCTTGTACGTTAGTG
AAGACGAACTCTGAAAGAAACTTCTCTGTTTTTGAAATGAGATTCTCCGGTGAGTACTCT
TCCGTCAT

Last Updated on 06.12.2018 16:11