Sequences from confirmation amplicon


Confirmation sequences for line 348B11 and hit At1g71950 are shown.
Line ID 348B11
Locus At1g71950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27081319
Primers used for PCR 27tv - 8474, show primer details
   
Sequence Name 20-K116964-0022-348-B11C-27tv
GenBank Accession LN495380 [GenBank]
Sequencing primer gene specific primer, 27tv: 5'-TATGGATTAACCATTTGAGACCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116964-0022-348-B11C-27tv
AATGGAAATGTCACCTCTCACGCGTTATTGAAAAAAAATTCCCAGATTCTATAAAAAAGA
GAAATGCAAACATTTGCTCCTGGAACAAGAGTCTACCACCTCATCATCATCGATCTTCTT
CTTCTTCTTCTGCTTATCATCGTTCTTCGCTTCAATCGTCATGGCTGATGAATATACCGG
AGAAGCAACGGGATCGTCGGAAGCAAAGGTTCACATCATCTACACCGAGAAACCTACCGA
TGAAGAACCTAAAACCTATGATCTCCGTACCCTTTCCTCTGCTCTCGGCAGGTCTGTAAT
CAAATTGCAACTCTCGATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 02-K116963-0022-348-B11C-8409
GenBank Accession LN487203 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K116963-0022-348-B11C-8409
TTGAATCGAGAGTTGCAATTTGATTACAGACCTGCCGAGAGCAGAGGAAAGGGTACGGAG
ATGATAGGTTTTAGGTTCTTCATCGGTAGGTTTCTCGGTGTAGATGATGTGAACCTTTGC
TTCCGACGATCCCGTTGCTTCTCCGGTATATTCATCAGCCATGACGATTGAAGCGAAGAA
CGATGATAAGCAGAAGAAGAAGAAGAAGACGATGATGATGAGGTGGTAGACTCTTGTTCC
AGGAGCAAATGTTTGCATTTCTCTTTTTTATAGAATCTGGGAATTTTTTTTCAATAACGC
GTGAGAGGTGACATTTTCCATTTATGTCACAATTTAAACCGACTCATAAATGGACGGTCT
CAAATGGTTTATCCAT

Last Updated on 06.12.2018 16:11