Sequences from confirmation amplicon


Confirmation sequences for line 348B11 and hit At4g00960 are shown.
Line ID 348B11
Locus At4g00960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:415268
Primers used for PCR PC77 - 8474, show primer details
   
Sequence Name 77-K103791-0022-348-B11C-PC77
GenBank Accession LN513540 [GenBank]
Sequencing primer gene specific primer, PC77: 5'-ATCAGGACAAGGAGACAATGAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K103791-0022-348-B11C-PC77
GAACTTCACATCGTAATCTTGTTAGGCTTTTAGGTTTCTGTTTAAAGGAGAAGAACGACT
TCTCATCTATGAGTTCTTTAAGAACACAAGCCTCGAAAAGTTCATATTTGGTACCTTAAA
ACACTAAACGCAAGCATATCTATCTCTAGTAACTAATAAAAATGAATTTCTCATACTTAT
ATCGTTTTCTTTTTTCAGATTCTGATAGAAGAATGATCTTAGATTGGGAAAAACGTTATA
GGATAATCTCAGGTGTTGCTCGAGGTCTTCTCTATCTCCATGAAGATTCTCATTTCAAAA
TTATTCATAGAGATATGAAAGCTAGCAATGTTCTATTGGACGATGCAATGAATCCGAAAA
TCGCAGATTTTGGGATGGTAAAATTGTTTAATACTGACCAAACAAGTCAGACCATGTTTA
CAAGCAAAGTTGCAGGAACTTAGTAAGCTGCCCAAAAACCATTTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
   
Sequence Name 93-K103792-0022-348-B11C-8409
GenBank Accession LN515252 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K103792-0022-348-B11C-8409
GCCATTTACATTGAAATGGTTTTTGGGCAGCTTACTAAGTTCCTGCAACTTTGCTTGTAA
ACATGGTCTGACTTGTTTGGTCAGTATTAAACAATTTTACCATCCCAAAATCTGCGATTT
TCGGATTCATTGCATCGTCCAATAGAACATTGCTAGCTTTCATATCTCTATGAATAATTT
TGAAATGAGAATCTTCATGGAGATAGAGAAGACCTCGAGCAACACCTGAGATTATCCTAT
AACGTTTTTCCCAATCTAAGATCATTCTTCTATCAGAATCTGAAAAAAGAAAACGATATA
AGTATGAGAAATTCATTTTTATTAGTTACTAGAGATAGATATGCTTGCGTTTAGTGTTTT
AAGGTACCAAATATGAACTTTTCGAGGCTTGTGTTCTTAAAGAACTCATAGATGAGAAGT
CGTTCTTCTCCTTTAAAACAGAAACCTAAAAGCCTAACAAGATTACGATGTTGAAGTTTC
GCAACCAATGAGACTTCGTTTACGAACTCATTGTCTCCTTGTCCTGA

Last Updated on 06.12.2018 16:11