Sequences from confirmation amplicon


Confirmation sequences for line 348E03 and hit At5g12270 are shown.
Line ID 348E03
Locus At5g12270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3970771
Primers used for PCR GC68 - 8474, show primer details
   
Sequence Name 86-K033712-022-348-E03C-GC68
GenBank Accession LN514608 [GenBank]
Sequencing primer gene specific primer, GC68: 5'-CAAAAGAATGGAGTGAAAGGACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K033712-022-348-E03C-GC68
CCTTCCTTCTCATACTTCACCTCCTCAGAACGCTTCACTTCCGACAAGATCCTCCTCGGA
TCACCTGTCCCGGTCATTGACGTGTCCAATTGGAATGAACCGCACGTGGCTAGAGAGATC
TGCCATGCAGCGTCAAAGCTCGGCTTGTTTCAGATAGTGAACCACGGGATAGCTCCGGCT
GAGTTCAAGGGTGTGATTGCTGCGGCGCGTGGGTTTTTCGAGTTGCCGGCGGAGGAGAGA
AGAAGGTATTGGAGAGGGAGTTCAGTGTCGGAGACGGCGTGGTTGACCACAAGTTTCAAT
CCTTGCATAGAAAGTGTTTTGGAATGGAGAGATTTCCTCAAGTTTGAGTATCTTCCTCAA
CGACATGACTTCGCTGCCACGTGGCCTTCTGTCTGCAAGTAAGTATAGTTACTGTGTTAC
TAAGTACATAATAGTACGTAATTACTGTATTGGCCTTCTGTGCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
CAC
   
Sequence Name 70-K033712-022-348-E03C-8409
GenBank Accession LN512320 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K033712-022-348-E03C-8409
TCGGACTGAGCCTTTACATTGAATATATCCTGCACAGAAGGCCAATACAGTAATTACGTA
CTATTATGTACTTAGTAACACAGTAACTATACTTACTTGCAGACAGAAGGCCACGTGGCA
GCGAAGTCATGTCGTTGAGGAAGATACTCAAACTTGAGGAAATCTCTCCATTCCAAAACA
CTTTCTATGCAAGGATTGAAACTTGTGGTCAACCACGCCGTCTCCGACACTGAACTCCCT
CTCCAATACCTTCTTCTCTCCTCCGCCGGCAACTCGAAAAACCCACGCGCCGCAGCAATC
ACACCCTTGAACTCAGCCGGAGCTATCCCGTGGTTCACTATCTGAAACAAGCCGAGCTTT
GACGCTGCATGGCAGATCTCTCTAGCCACGTGCGGTTCATTCCAATTGGACACGTCAATG
ACCGGGACAGGTGATCCGAGGAGGATCTTGTCGGAAGTGAAGCGTTCTTGAGGAGGTTGA
ATGTATGGAGAAGGAAGTGTGGTTAGGGTTAGAAAGTCGACGAGTCCTTTCACTCCATTC
TTTTG

Last Updated on 06.12.2018 16:11