Sequences from confirmation amplicon


Confirmation sequences for line 348G08 and hit At2g37200 are shown.
Line ID 348G08
Locus At2g37200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15622584
Primers used for PCR GD54 - 8474, show primer details
   
Sequence Name 87-K033712-022-348-G08C-GD54
GenBank Accession LN514715 [GenBank]
Sequencing primer gene specific primer, GD54: 5'-AATAAAGGAAGGAGACACAGCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K033712-022-348-G08C-GD54
TCTCAACCTTGTGCAGTGATTCACGTTACATTGTCCAAGATCGTTGATGAGTACAGTTAT
GCCAGCAGAAGCAGATGCAGCTGCAAACGTGAGCGTCGATGTGACCTGCAACACAATAGG
GGATGAAAAAACCGTCAGTCACGATACTCCACTTACTAATTAACACATCAAAAATGAAAC
ATTGAACAACCAAGTGTGAGGTTGATTTAGTCCTTACTCCATCTCCAATAGTGAAACACT
GAACAACGGAGTGATTTCGAAGGCTTCTTCTCACCAGAAGAGCATAAGCATCGATAATAA
AAAGAGACAAGCTCCACAGGCTTTGCAGGCTAACCGCAAGAACCAAGCAACTATCACAGT
AACAGATAAGAGCTTGTCAGATTCATATAGTCTAAAACCAAATCTAATAATTTTTAAGAC
ATTACACTAATAATTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTC
   
Sequence Name 71-K033712-022-348-G08C-8409
GenBank Accession LN512500 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K033712-022-348-G08C-8409
TTACAATTGAAATATCCTGAAATTATTAGTGTAATGTCTTAAAAATTATTAGATTTGGTT
TTAGACTATATGAATCTGACAAGCTCTTATCTGTTACTGTGATAGTTGCTTGGTTCTTGC
GGTTAGCCTGCAAAGCCTGTGGAGCTTGTCTCTTTTTATTATCGATGCTTATGCTCTTCT
GGTGAGAAGAAGCCTTCGAAATCACTCCGTTGTTCAGTGTTTCACTATTGGAGATGGAGT
AAGGACTAAATCAACCTCACACTTGGTTGTTCAATGTTTCATTTTTGATGTGTTAATTAG
TAAGTGGAGTATCGTGACTGACGGTTTTTTCATCCCCTATTGTGTTGCAGGTCACATCGA
CGCTCACGTTTGCAGCTGCATCTGCTTCTGCTGGCATAACTGTACTCATCAACGATCTTG
GACAATGTAACGTGAATCACTGCACAAGGTTTGAGACTGCCACAGCAATGGCCTTCATCA
GCTGGTTTGCTGTGTCTCCTTCCTTTAT

Last Updated on 06.12.2018 16:11