Sequences from confirmation amplicon


Confirmation sequences for line 348H06 and hit At5g59820 are shown.
Line ID 348H06
Locus At5g59820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24103582
Primers used for PCR BE67 - 8474, show primer details
   
Sequence Name 72-K028751-022-348-H06C-8409
GenBank Accession LN512647 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K028751-022-348-H06C-8409
CCTTTAAAATTTTTCTGAAATAATATTTTGTTTTCTCTCATTCTTTGAAATTTTTTCTTA
ATATTCTAGATTATACATACATCCGCAGATTTAGGAAACTTTCATAGAGTGTAATCTTTT
CTTTCTGTAAAAATATATTTTACTTGTAGCATTGGAGATTTGTTATGAGATTATCTTACT
TAGCATTTAGTGAATAATCTATTAGCCTATTTTGCCGACGTGAAAATACCATTTTCTATT
TAATTTTGATTACTTATCAACTACTATTAAGCTGTTATTTTCCCCCATCACGAATAAAAA
CAACCCAAGAAATAGATATTTATTTGAATTTGAAACTTACCTATAAATTTAGTTGAGCTA
ATCAAAATTAAATTTTAAAGCATGAT
   
Sequence Name 72-K028751-022-348-H06C-BE67
GenBank Accession LN512648 [GenBank]
Sequencing primer gene specific primer, BE67: 5'-AGAATTCCACTTCTGATCATGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K028751-022-348-H06C-BE67
AAATAAATATCTATTTCTTGGGTTGTTTTTATTCGTGATGGGGGAAAATAACAGCTTAAT
AGTAGTTGATAAGTAATCAAAATTAAATAGAAAATGGTATTTTCACGTCGGCAAAATAGG
CTAATAGATTATTCACTAAATGCTAAGTAAGATAATCTCATAACAAATCTCCAATGCTAC
AAGTAAAATATATTTTTACAGAAAGAAAAGATTACACTCTATGAAAGTTTCCTAAATCTG
CGGATGTATGTATAATCTAGAATATTAAGAAAAATTCAAAGAATGAGAGAAACAAATATA
TTCAGAAAATTTAAGGAAAATAAATAAAATCAATAAACTGTTCTTCCAAGCTCCAACTTG
AGATTCAAATTGTCCACCATCCCTAGACTCAGATCCAAACAAGCCACTCTCTTCCCACTG
CTAGATTTCTTCAACGTAGTCACCGTGGGCTCCGGCAACAAAGCGCGTGTAACCAACGCG
CCACCAGCAGCCCATATGTAGATTGGACAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTA

Last Updated on 06.12.2018 16:11