Sequences from confirmation amplicon


Confirmation sequences for line 349A04 and hit At3g07520 are shown.
Line ID 349A04
Locus At3g07520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2397111
Primers used for PCR AG08 - 8474, show primer details
   
Sequence Name 78-K105397-0022-349-A04C-AG08
GenBank Accession LN513692 [GenBank]
Sequencing primer gene specific primer, AG08: 5'-AGTTCCAGTGATTTCTTCTTTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K105397-0022-349-A04C-AG08
TTACTTTATTCAGCTACTCATGATACATCCTCTGAAGCAAAGGGAATTGCAGCTCTGTTC
AGCAATTTTGATTGGAGAACCGCTGTTCTTATCTACGAGGATGATGATGACTGGAGAGAG
AGTATACAACCTTTGGTTGGACATTTCCAACAGAACGCCATTCACATTGAGTACAAGGCG
GAATTTTCTGTCTCATCTAATGAGGAATGTATCATGAAGCAGCTAAGAAAGTTTAAGGCC
TCGGGGATAAGGATTTTTGTTGCGCACATTTCTGAACGAATTGCAAATCGTCTCTTTCCA
TGTGCTCGGAGGTTAGGAATGATGGAGGAAGGATATGCTTGGATCCTCACAGCGAGAAGC
ATGAATAATTTTCAAGACACAAATTATTTAGCAAAAGAGGAAATGGAAGGTGTGATTGGT
TTCAAGTCTTATATTCCGTTGACTGAGGAGCTTCACAACTTTACTTTGAGATGGAAGAGA
TCTTTGCGACTCGAAGAAGTAGTTACCAATTTTAAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 70-K105398-0022-349-A04C-8409
GenBank Accession LN512390 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K105398-0022-349-A04C-8409
TTACATTTAAATTGGTAACTACTTCTTCGAGTCGCAAAGATCTCTTCCATCTCAAAGTAA
AGTTGTGAAGCTCCTCAGTCAACGGAATATAAGACTTGAAACCAATCACACCTTCCATTT
CCTCTTTTGCTAAATAATTTGTGTCTTGAAAATTATTCATGCTTCTCGCTGTGAGGATCC
AAGCATATCCTTCCTCCATCATTCCTAACCTCCGAGCACATGGAAAGAGACGATTTGCAA
TTCGTTCAGAAATGTGCGCAACAAAAATCCTTATCCCCGAGGCCTTAAACTTTCTTAGCT
GCTTCATGATACATTCCTCATTAGATGAGACAGAAAATTCCGCCTTGTACTCAATGTGAA
TGGCGTTCTGTTGGAAATGTCCAACCAAAGGTTGTATACTCTCTCTCCAGTCATCATCAT
CCTCGTAGATAAGAACAGCGGTTCTCCAATCAAAATTGCTGAACAGAGCTGCAATTCCCT
TTGCTTCAGAGGATGTATCATGAGTAGCTTGAATAAAGTAATTATATTTGGCCAAGCTCA
ACGAGCTAGGAACTTGGAAAGAA
Primers used for PCR AG09 - 8474, show primer details
   
Sequence Name 51-K118809-0022-349-A04C-AG09
GenBank Accession LN507649 [GenBank]
Sequencing primer gene specific primer, AG09: 5'-ATTTTCGGAATTCTTGTAGACCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K118809-0022-349-A04C-AG09
ACGTCTTCTTCTATTGCTGATGAAACTACCAGAATTCCAAAGTCCTACACTTCTCTCACC
TCTCCCTATCATATTCACAATCTCAAACTTGTCTGAGATAAACTTCTTATCGATGAATTT
GATGTCACCACTCAAACCTTTATGCTTAGCACTCTCTGGAATAGCCTCAAGCAGGTCATA
TACTGACAAACCTGGTAACTTTGCAACTTCTGCTGCTCTTGCAAGACTCCAAGCTATATC
GTGAGCCCATATGCTACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCG
   
Sequence Name 19-K118808-0022-349-A04C-8409
GenBank Accession LN494977 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118808-0022-349-A04C-8409
ATTTACAATTGTAGCATATGGGCTCACGATATAGCTTGGAGTCTTGCAAGAGCAGCAGAA
GTTGCAAAGTTACCAGGTTTGTCAGTATATGACCTGCTTGAGGCTATTCCAGAGAGTGCT
AAGCATAAAGGTTTGAGTGGTGACATCAAATTCATCGATAAGAAGTTTATCTCAGACAAG
TTTGAGATTGTGAATATGATAGGGAGAGGTGAGAGAAGTGTAGGACTTTGGAATTCTGGT
AGTTTCATCAGCAATAGAAGAAGACGTTTGTCTTCTACCAAAGCACTTGAGACAATCATC
TGGCCTGGAGGGTCTACAAGAATTCCGAAAA

Last Updated on 06.12.2018 16:11