Genotyping and primer details


Genotyping and primer details for 349A04 - At3g07520
Line ID 349A04
Locus At3g07520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2397111 of line 349A04 Chr3:2397111
   
Insertion allele amplicon 8474 - AG08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AG08: 5'-AGTTCCAGTGATTTCTTCTTTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2397687
   
Insertion allele amplicon 8474 - AG09
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AG09: 5'-ATTTTCGGAATTCTTGTAGACCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2396788
   
wt amplicon (over InsPos) AG09 - AG08
wt amplicon start Chr3:2396788
wt amplicon end Chr3:2397687
wt amplicon size 900 bp
Locus specific primer AG08: 5'-AGTTCCAGTGATTTCTTCTTTCCA-3'
2nd locus specific primer AG09: 5'-ATTTTCGGAATTCTTGTAGACCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11