Sequences from confirmation amplicon


Confirmation sequences for line 349A06 and hit At5g43810 are shown.
Line ID 349A06
Locus At5g43810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17611753
Primers used for PCR PH59 - 8474, show primer details
   
Sequence Name 05-K017549-022-349-A06C-PH59
GenBank Accession LN488148 [GenBank]
Sequencing primer gene specific primer, PH59: 5'-GTTTGAGTTTTTACGAACACCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K017549-022-349-A06C-PH59
CAGCGACAATCTCACCGGCGGTGGCGACGGAGACGGCGGAGGAAGTGGTTTGACTTGGCG
GTGGCGGTTTACGAGGACGAGAGCTAGGTCTCATACAAACATCTCCTTGATCAGATTTTC
TTCCACCACGGTTTCTCCTCCGGCTACGATTCTTTGACGGTGGTGAAGGTGAAGAAGCTT
GACTCTGAGTAACCGTCGCCGGAGTAGTCACCGTCACCGGAGAAGGAGAAGGAGGAGGGA
TTTTACCGTTTTGAACGGTTTTTGGATTGTGGTGCTTTAAAGGTTGGGTTTTGATAACTA
AGTGAGTCTCAAAGCTATCTTTCATTTGGCCTAATCGGGAATTTTT
   
Sequence Name 06-K017549-022-349-A06C-8409
GenBank Accession LN488591 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K017549-022-349-A06C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATGACATTTTTGATTCTGGA
TTTTCTCCATTTAATGCAATCTGACATTTTCTTATAATTATTTTTTGTTTGTACTTATTT
TTCTTTCATCCTCATGATTTTGCAGGTAGAATAAGGCCAAGGAAGGGATCAGTTTTAAGT
TTTGTTTCATTCTTTTTGTAGTGGAGAAAAAGAGTTTTTGAAAATCCAAACAACAAAAAA
TGCCGATTAGGCAAATGAAAGATAGCTCTGAGACTCACTTAGTTATCAAAACCCAACCTT
TAAAGCACCACAATCCAAAAACCGTTCAAAACGGTAAAATCCCTCCTCCTTCTCCTTCTC
CGGTGACGGTGACTACTCCGGCGACGGTTACTCAGAGTCAAGCTTCTTCACCTTCACCAC
CGTCAAAGAATCGTAGCCGGAGGAGAAACCGTGGTGGAAGAAAATCTGATCAAGGAGATG
TTTGTATGAGACCTAGCTCTCGTCCTCGTAAACCGCCACCGCCAAGTCAAACCACTTCCT
CCGCCGTCTCCGTCGCCACCGCCGGTGAAATTGTCGCTGTGAATCATCAGATGCAGATGG
GTGTTCGTA

Last Updated on 06.12.2018 16:11