Sequences from confirmation amplicon


Confirmation sequences for line 349H10 and hit At3g47580 are shown.
Line ID 349H10
Locus At3g47580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17534084
Primers used for PCR EQ30 - 8474, show primer details
   
Sequence Name 47-K102871-0022-349-H10C-EQ30
GenBank Accession LN506167 [GenBank]
Sequencing primer gene specific primer, EQ30: 5'-TGTCTTTCAAGGATTCACATTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K102871-0022-349-H10C-EQ30
CAACAATCTTGCTCTCCGCCGCCAGTAAAGCCTTGAACACAGTACCAAAACTACCTGACC
CAACCATATTGCTTGAAGAGAAGCCATTTGTCGCATTTCGTAGATCTCCATAGCTTATCT
TCTCATGGAAAATCTCCAACTTAGAAGGAACCAGGTTATTTGTCTGCTGGTTCTTCCTTC
TTTTTCTGAACCAACACAGAACCATCGAAGCTATGACCAACAGCAATAGCAAAGCTATGC
CTATGCTTACTAAAATTGCAACTTTCTTTAAATGAGAGGAATGCTTTGTCTCCACTGGTG
GTTCTTGGGCGAGGCATGGCTTTAGTTTCAGATCCTTGATGCCTCCACATAGGTTTTTGT
TTCCAAATACAAAAACTATTGTAGAGTTCTGAAAATTTCCTTTGGAAGGCACCTTTCCCG
TAAAATTGTTAATAGATAGATTGAGATATTCCAACTTGGAGAAGTTTGCAAAATATTCAG
GTATACTTCCCGATAGATCATTGTTTGACAAATCAACTCTTCTTACACCCATCAACCCTC
TTATATTTGGAATTGCTCCATCAAAAGAATTTCCTTGTAGAAAAAGTTGTTCC
   
Sequence Name 47-K102876-0022-349-H10C-OriL
GenBank Accession LN506168 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K102876-0022-349-H10C-OriL
AATATGCAAATCCAACCCTTGTTAACCTAAGCATGGAAGGTAACTCTTTGAGCGGCTCTC
TACCAAATGATATTGGAAGTCTACAAAATCTTGTAAAACTATCACTTGAGAACAATAAGT
TTTCAGGACATCTCCCACAAACCTTGGGAAATTGTCTCGCGATGGAACAACTTTTTCTAC
AAGGAAATTCTTTTGATGGAGCAATTCCAAATATAAGAGGGTTGATGGGTGTAAGAAGAG
TTGATTTGTCAAACAATGATCTATCGGGAAGTATACCTGAATATTTTGCAAACTTCTCCA
AGTTGGAATATCTCAATCTATCTATTAACAATTTTACGGGAAAGGTGCCTTCCAAAGGAA
ATTTTCAGAACTCTACAATAGTTTTTGTATTTGGAAACAAAAACCTATGTGGAGGCATCA
AGGATCTGAAACTAAAGCCATGCCTCGCCCAAGAACCACCAGTGGAGACAAAGCATTCCT
CTCATTTAAAGAAAGTTGCAATTTTAGTAAGCATAGGCATAGCTTTGCTATTGCTGTTGG
TCATAGCTTCGATGGTTCTGTGTTGGTTCAGAAAAAGAAGGAAGAACCAGCAGACAAATA
ACCTGGTTCCTTCTAAGTTGGAGATTTTCCATGAGAAGATAAGCTATGGAGATCTACGAA
ATGCGACAAATGGCTTCTCTTCAAGCAATATGGTTGGGTCAGGTAGTTTTGGTACTGTGT
TCAAGGCATTGCTCCCGACGGAGAGCAAGATTGTTGCAGTGAAAGTTCTAAACATGCAGA
GACGTGGAGCTATGAAGAGCTTTATGGCAGAATGTGAATCCTTGGAAAG
Primers used for PCR EQ29 - 8474, show primer details
   
Sequence Name 46-K102871-0022-349-H10C-EQ29
GenBank Accession LN505831 [GenBank]
Sequencing primer gene specific primer, EQ29: 5'-CTGCTACCAAACATTCGTGAGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K102871-0022-349-H10C-EQ29
TTTAACTCTACAAAAGTTTGGAATCAACAAAAACATGATGACAGGAGGTATATATCCGAA
CTTTGGAAAAGTACCGAGTTTGCAATACCTAGATCTTAGTGAGAATCCTCTAGGAAGTTA
CACTTTTGGAGATCTTGAATTTATTGATTCTTTGACTAATTGCACCCATTTGCAACTCTT
AAGTGTTGGTTACACTAGGCTTGGGGGTGCCTTGCCTACATCCATTGCCAATATGTCCAC
AGAACTCATCAGTTTAAACCTTATAGGAAACCACTTTTTTGGAAGCATTCCTCAAGATAT
TGGGAATCTCATAGGCCTACAAAGACTTCAGTTGGGAAAAAATATGTTGACCGGACCACT
CCCAACTTCTTTGGGAAAGCTTCTAAGATTGGGGTTATTAAGTCTATATTCAAATAGGAT
GTCAGGAGAGATTCCATCTTTTATAGGCAACCTAACTCAGTTAGAAATACTATATTTGTC
CAACAATAGTTTTGAGGGAATTGTTCCTCCGAGTCTCGGGAAGTGTAGTCACATGCTTGA
ATTTACTCATTGGGAAATCATGTCCGGGAAATCTACATGGATCAGCACTGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACTGATTTTTTTTCAAT
   
Sequence Name 46-K102876-0022-349-H10C-8409
GenBank Accession LN505832 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K102876-0022-349-H10C-8409
GATTTCCCAATGCGTAAATCAAGCATGTGACTACACTTCCCGAGACTCGGAGGAACAATT
CCCTCAAAACTATTGTTGGACAAATATAGTATTTCTAATTGAGTTAGGTTGCCTATAAAA
GATGGAATCTCTCCTGACATCCTATTTGAATATAGACTTAATAACCCCAATCTTAGAAGC
TTTCCCAAAGAAGTTGGGAGTGGTCCGGTCAACATATTTTTTCCCAACTGAAGTCTTTGT
AGGCCTATGAGATTCCCAATATCTTGAGGAATGCTTCCAAAAAAGTGGTTTCCTATAAGG
TTTAAACTGATGAGTTCTGTGGACATATTGGCAATGGATGTAGGCAAGGCACCCCCAAGC
CTAGTGTAACCAACACTTAAGAGTTGCAAATGGGTGCAATTAGTCAAAGAATCAATAAAT
TCAAGATCTCCAAAAGTGTAACTTCCTAGAGGATTCTCACTAAGATCTAGGTATTGCAAA
CTCGGTACTTTTCCAAAGTTCGGATATATACCTCCTGTCATCATGTTTTTGTTGATTCCA
AACTTTTGTAGAGTTGAAATATTGGAAAGTGTTGTTGGAATGGCTCCCACTAGATCATTT
TCTCCCAGATTTAACTCACGAATGGTTTGGTAGCAG

Last Updated on 06.12.2018 16:11