Sequences from confirmation amplicon


Confirmation sequences for line 350A06 and hit At3g28080 are shown.
Line ID 350A06
Locus At3g28080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10453437
Primers used for PCR JZ31 - 8474, show primer details
   
Sequence Name 24-K037808-022-350-A06C-JZ31
GenBank Accession LN496911 [GenBank]
Sequencing primer gene specific primer, JZ31: 5'-TTGGTTAATTTTTCTATTGACCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K037808-022-350-A06C-JZ31
ATTATTATTGATCGTGTTTATTAATTTATCGAGTATTAATTTATAGAGTTTCTACTATAT
TTTTATTATAAAAAGTGGTGTACCTGGAGAATAAAAGAAACAGAAACGAAGATGCCTTGT
ATGGTAAGAATAGCTCCTCCGATGAGCCAATCAGACTTTGACGATGACAACGGTGGAGAA
AGCTGACGGAAGTTTAGATATGGTGGAGAAGATGCGACAAAGACACGTGGACCGTGGTAG
AAAATCACCACAAATGCGCCTATTAGTGACAGTGCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 32-K037808-022-350-A06C-8409
GenBank Accession LN500496 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K037808-022-350-A06C-8409
TGTCACTAATAGGCGCATTTGTGGTGATTTTCTACCACGGTCCACGTGTCTTTGTCGCAT
CTTCTCCACCATATCTAAACTTCCGTCAGCTTTCTCCACCGTTGTCATCGTCAAAGTCTG
ATTGGCTCATCGGAGGAGCTATTCTTACCATACAAGGCATCTTCGTTTCTGTTTCTTTTA
TTCTCCAGGTACACCACTTTTTATAATAAAAATATAGTAGAAACTCTATAAATTAATACT
CGATAAATTAATAAACACGATCAATTAATAAATTATATCGGTTCTAAGTCGGGTCAATAG
AAAAATTAACCAAACCAAAAGATTAAGA

Last Updated on 06.12.2018 16:11