Sequences from confirmation amplicon


Confirmation sequences for line 350C02 and hit At1g59650 are shown.
Line ID 350C02
Locus At1g59650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21920467
Primers used for PCR AB58 - 8474, show primer details
   
Sequence Name 34-K105952-0022-350-C02C-AB58
GenBank Accession LN501422 [GenBank]
Sequencing primer gene specific primer, AB58: 5'-GACGAAGCAAAAGAATGTTAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K105952-0022-350-C02C-AB58
AGTCTCATCACAATCGGTTTCAAGGCGAGATTGGAATCAAACCATGCTTCATCAACACTT
GCTGTTTGATAAAGACAGTGAGACTAAGTCAGGTGAATAATTATAACTCTAATAGAGAGA
CTGAAGAATTCAGCTAAAGTGACAGAAGTCTCTGAGTGTCAAATTAACAACAGTTTCATG
TTGTTTCTTAGTTTGATCCTCACGATTCAATGCTCATCAAAGATGTTAACCATTTAGAGA
AAGCTTCACTCTTTTTGAGTAATAGACCTACAAATCAAATCTAACAACACTCTAACCACA
ATGTAATCCCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCC
   
Sequence Name 50-K105953-0022-350-C02C-8409
GenBank Accession LN507172 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K105953-0022-350-C02C-8409
GCCATTTACATTGGGGATTACATTGTGGTTAGAGTGTTGTTAGATTTGATTTGTAGGTCT
ATTACTCAAAAAGAGTGAAGCTTTCTCTAAATGGTTAACATCTTTGATGAGCATTGAATC
GTGAGGATCAAACTAAGAAACAACATGAAACTGTTGTTAATTTGACACTCAGAGACTTCT
GTCACTTTAGCTGAATTCTTCAGTCTCTCTATTAGAGTTATAATTATTCACCTGACTTAG
TCTCACTGTCTTTATCAAACAGCAAGTGTTGATGAAGCATGGTTTGATTCCAATCTCGCC
TTTGAAACCGATTGTGATGATGACTTTCATAGTGTTCAGGAAGGTACAAGCAAAAGCTTT
AACATTCTTTGGCCTTCGT
Primers used for PCR 03au - 8474, show primer details
   
Sequence Name 73-K118874-0022-350-C02C-03au
GenBank Accession LN512981 [GenBank]
Sequencing primer gene specific primer, 03au: 5'-TACTGGATTTGAAGATCGTGTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K118874-0022-350-C02C-03au
GTAAAGGAAACAACTTTTTTGTTAGCTTTCTCTCTCTACAGTTTTGGGTTTTTAAGAGAA
AGAGATAAAGGAGACAGCTTTCTCTCTCTGTCTCTCTCTAAAAGTTCTGGGTTTAGAAAG
AGATCTTTCTTATATGTTGAATTTGTGATCAGAGGTGAGAGAGATGGGAGGTTGCGTGTC
AACGCCTAAGAGCTGTGTAGGAGGTAAAATAAGGTCAAGCAAAAGGAGAAAGACTCGGAC
AAGACGAAAGATTCAGAAGAAGAGAGTTGTTTCTTCTTCTCGCTTATCTGACGGATCTTT
CGATAATAATCCCACTTTCAGAGGTCTCTCTCTCTCTCTCTTTCTCTAATCTCTAATCTC
TCATTTCATCAAAAATCCATCTTTTGTTCCTCTCTGCATTTCTCTATTGGTTTTGGTCTT
GCTCTGTGTTTTTTGATTGATTTGGCTTCGTGGGTTATTGTCTTTCTTGTGTATCTCGAA
GGTTTGTTTTAGCTTTTTGATTGATTGAATTCGAAATATGTTGTCTTTTGATTGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCC
   
Sequence Name 33-K118873-0022-350-C02C-8409
GenBank Accession LN501197 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K118873-0022-350-C02C-8409
ATTTACATTGGAATATATCCATCAAAAGACAACATATTTCGAATTCAATCAATCAAAAAG
CTAAAACAAACCTTCGAGATACACAAGAAAGACAATAACCCACGAAGCCAAATCAATCAA
AAAACACAGAGCAAGACCAAAACCAATAGAGAAATGCAGAGAGGAACAAAAGATGGATTT
TTGATGAAATGAGAGATTAGAGATTAGAGAAAGAGAGAGAGAGAGAGACCTCTGAAAGTG
GGATTATTATCGAAAGATCCGTCAGATAAGCGAGAAGAAGAAACAACTCTCTTCTTCTGA
ATCTTTCGTCTTGTCCGAGTCTTTCTCCTTTTGCTTGACCTTATTTTACCTCCTACACAG
CTCTTAGGCGTTGACACGCAACCTCCCATCTCTCTCACCTCTGATCACAAATTCAACATA
TAAGAAAGATCTCTTTCTAAACCCAGAACTTTTAGAGAGAGACAGAGAGAGAAAGCTGTC
TCCTTTATCTCTTTCTCTTAAAAACCCAAAACTTGTAGAGAGAGAAAGCTAACAAAAAAG
TTGTTTCCTTTTACTTCTCTCTGATCAGAAAATCAGATAAAATCTGCCAGACACGATTC

Last Updated on 06.12.2018 16:11