Sequences from confirmation amplicon


Confirmation sequences for line 350F01 and hit At1g68990 are shown.
Line ID 350F01
Locus At1g68990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25938044
Primers used for PCR MI33 - 8474, show primer details
   
Sequence Name 34-K032520-022-350-F01C-MI33
GenBank Accession LN501306 [GenBank]
Sequencing primer gene specific primer, MI33: 5'-GAATGTCACAAAAGTTGCTCATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K032520-022-350-F01C-MI33
TGGTGGATGGGGATATGGGATATTGCAGCTTCAGGAAAGGGGCTTCTCAAGGCTTCTGAA
AGATTAATGCAAGCAGCCAAACATTGAAAGGGATCCTCTGCGTTAAGCCACCATCTTTTT
CCTTCAAGAGGTCTATCAGAAGAATCAAATATATCTTCCAAGTGACTCTCAGTAAACGCA
ATCCTATCCTCATAAGCCAGCTTATCGACTCCACCAGCATATAAGTTTGCTATATGTATC
TTCAGCCAACGCAACCCTGATTTCCCAAGAGGCTTTCCCTCACAAAACTCAAGGATGCCT
CGGCAGAGTTAATATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACTT
   
Sequence Name 34-K032520-022-350-F01C-8409
GenBank Accession LN501305 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K032520-022-350-F01C-8409
ATTAACTCTGCCGAGGCATCCTTGAGTTTTGTGAGGGAAAGCCTCTTGGGAAATCAGGGT
TGCGTTGGCTGAAGATACATATAGCAAACTTATATGCTGGTGGAGTCGATAAGCTGGCTT
ATGAGGATAGGATTGCGTTTACTGAGAGTCACTTGGAAGATATATTTGATTCTTCTGATA
GACCTCTTGAAGGAAAAAGATGGTGGCTTAACGCAGAGGATCCCTTTCAATGTTTGGCTG
CTTGCATTAATCTTTCAGAAGCCTTGAGAAGCCCCTTTCCTGAAGCTGCAATATCCCATA
TCCCCATCCACCAGGTACTCGCTAGATTTTATCCATACTTGTACATGAGCAACTTTT
Primers used for PCR FP51 - 8474, show primer details
   
Sequence Name 62-K035339-022-350-F01C-8409
GenBank Accession LN510687 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K035339-022-350-F01C-8409
TGAGCCATTTACAATTGAATATATCCTCGCCCACGAAAATCAACGTTGTGAGGGTAGTAG
AAACCTTCCTCATCCTTCATTTTACGTGCCACCTGATCAATCAATTGAAAGCACAACGAT
GGTTTTATACATCACAGCAGCAAAAAAGAGTGCAAAAAGAAGTCTTCTCTTACCTCGAGT
TTGAGCTCTATATCACACCTTTGTGAATGTCTCTCATTATTTTGCTTAATTGCTTTTTTG
GATTCCCATCTCCAATTTTTAAATTTTTCTTGGTCTTCCCTTTCCGGCTCTTCTGGAATG
GGTACCTTTTGTAGTTTATAAAGAGGTGAGAAAAAATCAAAATTTCTAAAAGAGAAGTAG
GAATCAAAATTACTTCCTTCTTCTGAAGAAGATGGCTAGCGGATGGGTGAATAAGAACTA
CAAGTAGATCCGCAAAGAAGAGAGGGAGAGATCTTACATCTTCACGGTCAACCAGACCAC
CAATGCGACCTCCATTAGCCCAGATTCTGTCCACCAAACTTAA
   
Sequence Name 62-K035339-022-350-F01C-FP51
GenBank Accession LN510688 [GenBank]
Sequencing primer gene specific primer, FP51: 5'-GGTGTTAAGTTTGGTGGACAGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K035339-022-350-F01C-FP51
AGTGCATTGGTGGTCTGGTTGACGTGAAGATGTAAGATCTCTCCCTCTCTTCTTTGCGGA
TCTACTTGTAGTTCTTATTCACCCATCCGCTAGCCATCTTCTTCAGAAGAAGGAAGTAAT
TTTGATTCCTACTTCTCTTTTAGAAATTTTGATTTTTTCTCACCTCTTTATAAACTACAA
AAGGTACCCATTCCAGAAGAGCCGGAAAGGGAAGACCAAGAAAAATTTAAAAATTGGAGA
TGGGAATCCAAAAAAGCAATTAAGCAAAATAATGAGAGACATTCACAAAGGTGTGATATA
GAGCTCAAACTCGAGGTAAGAGAAGACTTCTTTTTGCACTCTTTTTTGCTGCTGTGATGT
ATAAAACCATCGTTGTGCTTTCAATTGATTGATCATGTGGCACGTATAATGAAGGATGAG
GAAGGGTTCTACTACCCTCACAACGTTGATTTTCGGTGGGCGAGGATATAATCCATTGGT
AAATGGCTTCCTGTCCGGGGA

Last Updated on 06.12.2018 16:11