Sequences from confirmation amplicon


Confirmation sequences for line 350G06 and hit At3g18730 are shown.
Line ID 350G06
Locus At3g18730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6452838
Primers used for PCR 75za - 3144, show primer details
   
Sequence Name 21-K123937-0022-350-G06C-75za
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 75za: 5'-TTCATCATTAAAAACCCCTACGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K123937-0022-350-G06C-75za
ACTTTCAATAATGCGAACATGGGGTGGAGTGATAGGACTAAAAAGGAAGGAGTGTTTAAG
TTATAAAACTCACCTTCAGCCATGCGAATACCATGCTAGATTTATCAATAAGACTTCCAA
GACAAGCATTTACTTGGAGCATAGACTTTCGTTCCTCCGAAGTGCCTTTGGCATCAGTCA
TTTCTGCAGACAACTTCTTAAGCATAAGCTCTTCTTCTCTCAATTCTTCCATAACTTTCA
TGGATTTCTTGACTATCTTGGTATTATGCTCTATCTGTTCAACCAATGCACTCTCGTCTT
GCATAGATTTCGCTAGACTAGAAGCTTTACCATAACATAAAAGAGCATCAATGTACTTTT
GGGTCTTGTTGTGTAATTCAGCGAGATTGATATACCCCTTCGCTTCTCCTTGGACATGAT
TAATCTTATGACAGATATTAATATCCATCTCAATGTGTTTCTTTGCTTCATCCCAACTTC
TCAGCGCCATAAAAACGTTTCCAAGGTTATGATGAAGCCTACTCCGAGCAGCATCATACT
CTCTCACCTCCTCTTCATCGCAAATCTGCAGCCCTTTCTTAAGAATAGTACGGGCTGCTT
CAGGATTATCAAGATCAAGGTCAAGCATACCGATGTTGTTATGTGCGTTAATATACTCCT
CAAGGAATCCGCTAGATTCTCCAGGAGGTGGTTTCTCCTTGAGAATCTGTGCAAGTTCCA
TGGCTTTCTTAAAGTACTTTTTAGCACTCTGAATGGCTTCACAATCATCCTCAGACTTCA
AGAACATTTCATGGTAAGTACGTCCAAGTTGAGTACATGCTCTTTGCTTCTCCACAGTGT
CATTAGCTTCTTCAGCTAGCTGTAAATGCTTCTTCTGCAACCCCAGAAAAAAACACAAAT
TCAAAATGAAAAA
   
Sequence Name 05-K123936-0022-350-G06C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K123936-0022-350-G06C-3144
CAGTCTCTTGGCGAGATCTATCTCCGCCTCGAAAATTTCCCATAAGCCTTGATTTATCAG
GTAAGCCCTCTTGAATCAATTGCTTTTTCCTACTTGGTTATTGTTGGCTTCCTGAATTTT
CCGTGAATAATTTTGGTGTTTGAGTTTTTCATTTTGAATTTGTGTTTTTTTCTGGTGGTT
GCAGAAGAAGCATTTACAGCTAGCTGAAGAAGCTAATGACACTGTGGAGAAGCAAAGAGC
ATGTACTCAACTTGGACGTACTTACCATGAAATGTTCTTGAAGTCTGAGGATGATTGTGA
AGCCATTCAGAGTGCTAAAAAGTACTTTAAGAAAGCCATGGAACTTGCACAGATTCTCAA
GGAGAAACCACCTCCTGGAGAATCTAGCGGATTCCTTGAGGAGTATATTAACGCACATAA
CAACATCGGTATGCTTGACCTTGATCTTGATAATCCTGAAGCAGCCCGTACTATTCTTAA
GAAAGGGCTGCAGATTTGCGATGAAGAGGAGGTGAGAGAGTATGATGCTGCTCGGAGTAG
GCTTCATCATAACCTTGGAAACGTTTTTATGGCGCTGAGAAGTTGGGATGAAGCAAAGAA
ACACATTGAGATGGATATTAATATCTGTCATAAGATTAATCATGTCCAAGGAGAAGCGAA
GGGGTATATCAATCTCGCTGAATTACACAACAAGACCCAAAAGTACATTGATGCTCTTTT
ATGTTATGGTAAAGCTTCTAGTCTAGCGAAATCTATGCAAGACGAGAGTGCATTGGTTGA
ACAGATAGAGCATAATACCAAGATAGTCAAGAAATCCATGAAAGTTATG

Last Updated on 06.12.2018 16:11