SimpleSearch - Line and FST details
Line specific information
| Line ID | 350G06 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N433582) |
| Segregation Analysis | 50:49:45 |
| Confirmed for Hit | At4g26910 At3g18730 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 7912 |
| Note |
Gene hit At4g26910
| Sequence (A. th genome BLAST matches underlined) | >47-K016245-022-350-G06-8409 TGATCTGAAATTTCCCGGACTGAAGCCATTTACATTGAATATATCCTGTGTCAATATAGG AACTTCCTGTGTCAAATAAGACTTTCCCTACTCTTCCGTTTTCCCCATCTAAGCTAAGCA TACCCTGCCCGTAGCTCATCTTTGTAACTTGCATCTGATAAGCATCCCTGAAAGAACCCA AAAATGTACATTACCTTAGCATATATGCAAGTGACAAAGATTGAAGCTTTTGGGAATATA TTCAGATAAGAATCCAATCAAGCGATTCTAAGCTGCTATGGGATCCCCCCTATAGTGAGA NATAGCGACCTTGGTTCCTGGTTCTACAGTATCTCCTTCGTTGGCTATGGGATCCTCCCT ATAGTGAGANTNNNNTGCNATNTTAAGTAGGGGGTGACAGATAGCTGTGCAACGGAACTC GAAGAGGCCTCCAGATGTTACCCTTTGCCCAACAGTCTCAATTGACCGTTGGTCTTCCGA GACGGTATCCCCTGATGTCGCTGGAAGTAAACCCCAGCGTCTTGCTCCACCATGTCGACG AGCATTTCCGTCTTGTCATTGAAGCCGCACGACATTCCGGATCAACTGCTAGCCCGGCTT TTACGGGGAGCTCTGT |
| GenBank Accession | AL953387 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr4:13521326 - go to primer design |
| BLAST e Value | 6e-15 |
| Hit Clone Code (BAC ID) | F10M23 |
| Hit Gene Code | At4g26910 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Dihydrolipoamide succinyltransferase |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At1g49050
| Sequence (A. th genome BLAST matches underlined) | >47-K016245-022-350-G06-8409 TGATCTGAAATTTCCCGGACTGAAGCCATTTACATTGAATATATCCTGTGTCAATATAGG AACTTCCTGTGTCAAATAAGACTTTCCCTACTCTTCCGTTTTCCCCATCTAAGCTAAGCA TACCCTGCCCGTAGCTCATCTTTGTAACTTGCATCTGATAAGCATCCCTGAAAGAACCCA AAAATGTACATTACCTTAGCATATATGCAAGTGACAAAGATTGAAGCTTTTGGGAATATA TTCAGATAAGAATCCAATCAAGCGATTCTAAGCTGCTATGGGATCCCCCCTATAGTGAGA NATAGCGACCTTGGTTCCTGGTTCTACAGTATCTCCTTCGTTGGCTATGGGATCCTCCCT ATAGTGAGANTNNNNTGCNATNTTAAGTAGGGGGTGACAGATAGCTGTGCAACGGAACTC GAAGAGGCCTCCAGATGTTACCCTTTGCCCAACAGTCTCAATTGACCGTTGGTCTTCCGA GACGGTATCCCCTGATGTCGCTGGAAGTAAACCCCAGCGTCTTGCTCCACCATGTCGACG AGCATTTCCGTCTTGTCATTGAAGCCGCACGACATTCCGGATCAACTGCTAGCCCGGCTT TTACGGGGAGCTCTGT |
| GenBank Accession | AL953387 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:18152374 - go to primer design |
| BLAST e Value | 2e-123 |
| Hit Clone Code (BAC ID) | F27J15 |
| Hit Gene Code | At1g49050 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Eukaryotic aspartyl protease family protein |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
| Other FSTs Supporting this Hit | AL953388 [GenBank] |
Gene hit At3g18730
| Sequence (A. th genome BLAST matches underlined) | >10-K123792-0022-350-G06C-3144 TCAGCAAAGCTATTTGGTGATGTGTACAACTGGCTGACTGACCCATATTTCTTACAAGAA ACCACCTCCTGGAGAATCTAGCGGATTCCTTGAGGAGTATATTAACGCACATAACAACAT CGGTATGCTTGACCTTGATCTTGATAATCCTGAAGCAGCCCGTACTATTCTTAAGAAAGG GCTGCAGATTTGCGATGAAGAGGAGGTGAGAGAGTATGATGCTGCTCGGAGTAGGCTTCA TCATAACCTTGGAAACGTTTTTATGGCGCTGAGAAGTTGGGATGAAGCAAAGAAACACAT TGAGATGGATATTAATATCTGTCATAAGATTAATCATGTCCAAGGAGAAGCGAAGGGGTA TATCAATCTCGCTGAATTACACAACAAGACCCAAAAGTACATTGATGCTCTTTTATGTTA TGGTAAAGCTTCTAGTCTAGCGAAATCTATGCAAGACGAGAGTGCATTGGTTGAACAGAT AGAGCATAATACCAAGATAGTCAAGAAATCCATGAAAGTTATGGA |
| GenBank Accession | queueing for submission to ENA/GenBank |
| Graphic View |
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| Predicted Position of Insertion | Chr3:6452476 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | MVE11 |
| Hit Gene Code | At3g18730 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | tetratricopeptide repeat (TPR)-containing protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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