Sequences from confirmation amplicon


Confirmation sequences for line 350G06 and hit At4g26910 are shown.
Line ID 350G06
Locus At4g26910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13521296
Primers used for PCR 25nt - 8474, show primer details
   
Sequence Name 14-K114570-0022-350-G06C-25nt
GenBank Accession LN492608 [GenBank]
Sequencing primer gene specific primer, 25nt: 5'-TTGCACAAATTGAAACTGATAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114570-0022-350-G06C-25nt
TTTTCTAAGACTCTGTGTTAATTTTAAAATCTATTATTTGATTCATATTTGTGGATTAGG
TGACAATAGATATTGCTAGCCCAGCAAGTGGTGTAATCCAAGAGGTAAATATGTTTGCTC
TTTGTGTTGTTTATTCAGTCGTTATTGTTGTTCTCTTTCTGGTGTCTCTCTATAGTTAGT
CTTCACTGACTAGACCTGTTTGATTTTGTCTTCTAATGCAGTTTCTAGTCAACGAAGGAG
ATACTGTAGAACCAGGAACCAAGGTCGCTATTATATCAAAGTCTGAGGATACTGCATCTC
AGGTTACCCCCTCTCAGAAGATACCAGAGACAACTGATACCAAACCTTCTCCTCCTGCTG
AAGATAAGCAGAAGCCCAGAGTGGAAAGTGCTCCTGTAGCAGAGAAACCCAAAGCACCAT
CCTCACCACCTCCACCCAAACAGTCTGCTAAAGAACCGCAGCTTCCTCCTAAGGAAAGAG
AAAGACGGGTATGCAAAATCTATCATTTTTCCTGGCTTCCGTCTGAAAATTTTTAAATGA
CTGATAAAAAGCTTTACAGTCACATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAC
GGTTATA
   
Sequence Name 01-K114569-0022-350-G06C-8409
GenBank Accession LN486675 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K114569-0022-350-G06C-8409
GCTTTTTTCANTCATTTAAAATTTTCAGACGGAAGCCAGGAAAAATGATAGATTTTGCAT
ACCCCGTCTTTCTCTTTCCTTAGGAGGAAGCTGCGGTTCTTTAGCAGACTGTTTGGGTGG
AGGTGGTGAGGATGGTGCTTTGGGTTTCTCTGCTACAGGAGCACTTTCCACTCTGGGCTT
CTGCTTATCTTCAGCAGGAGGAGAAGGTTTGGTATCAGTTGTCTCTGGTATCTTCTGAGA
CGGGGTAACCTGAGATGCACTATCCTCAGACTTTCATATAATAGCGACCTTGGTTCCTGG
TTCTACGTATCTCCTTCGTTGACTAGACACTGCATTACAATCAACATCAAACCGGTCTCG
TCAGTGAAGACTAACTATACAGACACACCAGACAGAGAACAACAACTAACGACTGAATAA
ACAACACACAGACCATACCTATTTACCTCTTGGATTACACCACTTGCTGGGCTAGCAATA
TCTATTGTCACCTAATCCACAAATATGAATCAAATCCTCCCTTTTACAATTCAACACAGA
TGTCTTTAGAAAAATCACATATAACACTCAATCTTGATACCTTATCAGTTTCAATTTGTG
C

Last Updated on 06.12.2018 16:11