Sequences from confirmation amplicon


Confirmation sequences for line 351D08 and hit At1g04880 are shown.
Line ID 351D08
Locus At1g04880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1378261
Primers used for PCR 27bk - 8474, show primer details
   
Sequence Name 23-K107548-0022-351-D08C-27bk
GenBank Accession LN496584 [GenBank]
Sequencing primer gene specific primer, 27bk: 5'-TACAATACATTACCCCCAAGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K107548-0022-351-D08C-27bk
AAAAGACATTACTTTTAGTCAGACTATACACACAGAAGAGGAATCCATAATATAGACAGA
GAATCTCATGCATTGTAACTGGTAAAATTTTTGTAGGTGAAAAACAAAGCATGATTCATC
GTCAACATAAGAGCTTAAATCTCAAAGATAGAGACTTTCTGAACACAAATTTCTGTCAGA
GCAGATAACAACTGAACTTACTCTACTAAGTAAGATATGATAAGGGAAGAAAGAAGCTTA
CATGAATTTAGTACCCAAGAGAGAGTGCAGCCTTTCCAAGGAAGTCATGAAAAGCCTAGG
GTCTGCAACCACAGCCTCATACGTTGCTTCAGGGGTAACACAAACATTGTTCATTGGCAC
TGACCCTTGCTTGAGACAAGAGCTTGATGCAGAATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATT
   
Sequence Name 07-K107547-0022-351-D08C-8409
GenBank Accession LN489301 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K107547-0022-351-D08C-8409
ATTTACAATTGAATATATTCTGCATCAAGCTCTTGTCTCAAGCAAGGGTCAGTGCCAATG
AACAATGTTTGTGTTACCCCTGAAGCAACGTATGAGGCTGTGGTTGCAGACCCTAGGCTT
TTCATGACTTCCTTGGAAAGGCTGCACTCTCTCTTGGGTACTAAATTCATGTAAGCTTCT
TTCTTCCCTTATCATATCTTACTTAGTAGAGTAAGTTCAGTTGTTATCTGCTCTGACAGA
AATTTGTGTTCAGAAAGTCTCTATCTTTGAGATTTAAGCTCTTATGTTGACGATGAATCA
TGCTTTGTTTTTCACCTACAAAAATTTTACCAGTTACAATGCATGAGATTCTCTGTCTAT
ATTATGGATTCCTCTTCTGTGTGTATAGTCTGACTAAAAGTAATGTTCTTTTTTGTGTAA
CGCATTGTTCCTTTTGCTTCTTGGGGGTAATGTATTGTA

Last Updated on 06.12.2018 16:11